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验证数据展示

  • WB analysis using 68268-1-Ig

    WB analysis using 68268-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68268-1-Ig (ZHX2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis of HEK-293 using 68268-1-Ig

    WB analysis of HEK-293 using 68268-1-Ig

    WB result of ZHX2 antibody (68268-1-Ig; 1:24000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZHX2 transfected HEK-293 cells.

  • IHC staining of mouse brain using 68268-1-Ig

    IHC staining of mouse brain using 68268-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68268-1-Ig (ZHX2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ZHX2 antibody included
  • IHC staining of mouse brain using 68268-1-Ig

    IHC staining of mouse brain using 68268-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68268-1-Ig (ZHX2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ZHX2 antibody included
  • IF Staining of A431 using 68268-1-Ig

    IF Staining of A431 using 68268-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using ZHX2 antibody (68268-1-Ig, Clone: 4E12F2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

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  • KD/KO验证

ZHX2 Monoclonal antibody

ZHX2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 68268-1-Ig

产品说明书

CloneNo. 4E12F2

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IHC, IF/ICC, ELISA

别名

RAF, KIAA0854, Alpha-fetoprotein regulator 1, AFR1, AFP regulator 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inSH-SY5Y cells, HEK-293 cells, LNCaP cells, HeLa cells, Jurkat cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy ZHX2 Ready-To-Use IHC Kit. ZHX2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68268-1-Ig targets ZHX2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原 ZHX2 fusion protein Ag14420 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 zinc fingers and homeoboxes 2
别名 RAF, KIAA0854, Alpha-fetoprotein regulator 1, AFR1, AFP regulator 1
计算分子量 837 aa, 92 kDa
观测分子量92-100 kDa
GenBank蛋白编号BC042145
基因名称 ZHX2
Gene ID (NCBI) 22882
RRIDAB_2935352
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9Y6X8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Zinc fingers and homeoboxes protein 2 is a protein that in humans is encoded by the ZHX2 gene. ZHX2 Act as a transcriptional repressor, represses the promoter activity of the CDC25C gene stimulated by NFYA (PMID:12741956). In addition to forming homodimers, this protein heterodimerizes with member 1 of the zinc fingers and homeoboxes family.

实验方案

Product Specific Protocols
WB protocol for ZHX2 antibody 68268-1-IgDownload protocol
IHC protocol for ZHX2 antibody 68268-1-IgDownload protocol
IF protocol for ZHX2 antibody 68268-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols