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  • Featured Product
  • KD/KO Validated

ZHX2 Monoclonal antibody, PBS Only

ZHX2 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4E12F2

Cat no : 68268-1-PBS

Print datasheet

Synonyms

RAF, KIAA0854, Alpha-fetoprotein regulator 1, AFR1, AFP regulator 1

验证数据展示

  • WB analysis using 68268-1-Ig (same clone as 68268-1-PBS)

    WB analysis using 68268-1-Ig (same clone as 68268-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68268-1-Ig (ZHX2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68268-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 68268-1-Ig (same clone as 68268-1-PBS)

    WB analysis of HEK-293 using 68268-1-Ig (same clone as 68268-1-PBS)

    WB result of ZHX2 antibody (68268-1-Ig; 1:24000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZHX2 transfected HEK-293 cells. This data was developed using the same antibody clone with 68268-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 68268-1-Ig (same clone as 68268-1-PBS)

    IF Staining of A431 using 68268-1-Ig (same clone as 68268-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using ZHX2 antibody (68268-1-Ig, Clone: 4E12F2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68268-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy ZHX2 Ready-To-Use IHC Kit. ZHX2 primary antibody included.

产品信息

68268-1-PBS targets ZHX2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig, rabbit
Immunogen ZHX2 fusion protein Ag14420 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name zinc fingers and homeoboxes 2
Synonyms RAF, KIAA0854, Alpha-fetoprotein regulator 1, AFR1, AFP regulator 1
Calculated Molecular Weight 837 aa, 92 kDa
Observed Molecular Weight92-100 kDa
GenBank Accession NumberBC042145
Gene Symbol ZHX2
Gene ID (NCBI) 22882
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9Y6X8
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Zinc fingers and homeoboxes protein 2 is a protein that in humans is encoded by the ZHX2 gene. ZHX2 Act as a transcriptional repressor, represses the promoter activity of the CDC25C gene stimulated by NFYA (PMID:12741956). In addition to forming homodimers, this protein heterodimerizes with member 1 of the zinc fingers and homeoboxes family.