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验证数据展示

  • WB analysis using 68281-1-Ig

    WB analysis using 68281-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68281-1-Ig (LZTFL1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • IF Staining of Jurkat using 68281-1-Ig

    IF Staining of Jurkat using 68281-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using LZTFL1 antibody (68281-1-Ig, Clone: 2C1F6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • FC experiment of Jurkat using 68281-1-Ig

    FC experiment of Jurkat using 68281-1-Ig

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human LZTFL1 (68281-1-Ig, Clone:2C1F6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

LZTFL1 Monoclonal antibody

LZTFL1 Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 68281-1-Ig

产品说明书

CloneNo. 2C1F6

宿主/亚型

Mouse / IgG2a

种属反应性

Human

应用

WB, IF/ICC, FC (Intra), ELISA

别名

Leucine zipper transcription factor-like protein 1, 2C1F6

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHCT 116 cells, LNCaP cells, HEK-293 cells, HeLa cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inJurkat cells
Positive FC (Intra) detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy LZTFL1 Ready-To-Use IHC Kit. LZTFL1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68281-1-Ig targets LZTFL1 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 Human
免疫原 LZTFL1 fusion protein Ag10733 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 leucine zipper transcription factor-like 1
别名 Leucine zipper transcription factor-like protein 1, 2C1F6
计算分子量 299 aa, 35 kDa
观测分子量 35 kDa
GenBank蛋白编号BC025988
基因名称 LZTFL1
Gene ID (NCBI) 54585
RRIDAB_2935362
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NQ48
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LZTFL1 belongs to the LZTFL1 family. It is an important regulator of BBSome ciliary trafficking and hedgehog signaling. LZTFL1 regulates Ciliary Trafficking of the BBSome and Smoothened.(PMID:22072986)

实验方案

Product Specific Protocols
WB protocol for LZTFL1 antibody 68281-1-IgDownload protocol
IF protocol for LZTFL1 antibody 68281-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols