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LZTFL1 Monoclonal antibody, PBS Only

LZTFL1 Monoclonal Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2C1F6

Cat no : 68281-1-PBS

Print datasheet

Synonyms

Leucine zipper transcription factor-like protein 1, 2C1F6

验证数据展示

  • WB analysis using 68281-1-Ig (same clone as 68281-1-PBS)

    WB analysis using 68281-1-Ig (same clone as 68281-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68281-1-Ig (LZTFL1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68281-1-PBS in a different storage buffer formulation.

  • IF Staining of Jurkat using 68281-1-Ig (same clone as 68281-1-PBS)

    IF Staining of Jurkat using 68281-1-Ig (same clone as 68281-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using LZTFL1 antibody (68281-1-Ig, Clone: 2C1F6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68281-1-PBS in a different storage buffer formulation.

  • FC experiment of Jurkat using 68281-1-Ig (same clone as 68281-1-PBS)

    FC experiment of Jurkat using 68281-1-Ig (same clone as 68281-1-PBS)

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human LZTFL1 (68281-1-Ig, Clone:2C1F6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68281-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy LZTFL1 Ready-To-Use IHC Kit. LZTFL1 primary antibody included.

产品信息

68281-1-PBS targets LZTFL1 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity Human
Immunogen LZTFL1 fusion protein Ag10733 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name leucine zipper transcription factor-like 1
Synonyms Leucine zipper transcription factor-like protein 1, 2C1F6
Calculated Molecular Weight 299 aa, 35 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC025988
Gene Symbol LZTFL1
Gene ID (NCBI) 54585
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NQ48
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

LZTFL1 belongs to the LZTFL1 family. It is an important regulator of BBSome ciliary trafficking and hedgehog signaling. LZTFL1 regulates Ciliary Trafficking of the BBSome and Smoothened.(PMID:22072986)