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验证数据展示

  • WB analysis using 68304-1-Ig

    WB analysis using 68304-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68304-1-Ig (ATP5F1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • IF Staining of HeLa using 68304-1-Ig

    IF Staining of HeLa using 68304-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ATP5F1 antibody (68304-1-Ig, Clone: 1A10G9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

ATP5F1 Monoclonal antibody

ATP5F1 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68304-1-Ig

产品说明书

CloneNo. 1A10G9

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Rat

应用

WB, IF/ICC, ELISA

别名

ATP5F1, ATPase subunit b, PIG47

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68304-1-Ig targets ATP5F1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, Mouse, Rat
免疫原 ATP5F1 fusion protein Ag8571 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ATP synthase, H+ transporting, mitochondrial F0 complex, subunit B1
别名 ATP5F1, ATPase subunit b, PIG47
计算分子量 256 aa, 29 kDa
观测分子量 25-30 kDa
GenBank蛋白编号BC005366
基因名称 ATP5F1
Gene ID (NCBI) 515
RRIDAB_2935383
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP24539
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATP5F1(ATP synthase subunit b) belongs to the eukaryotic ATPase B chain family. The ATP5F1 gene encodes subunit B of the mitochondrial ATP synthase Fo unit, which contains 214-amino acid with a 42-amino acid import signal(PMID:1831354). Mitochondrial membrane ATP synthase(F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain.

实验方案

Product Specific Protocols
WB protocol for ATP5F1 antibody 68304-1-IgDownload protocol
IF protocol for ATP5F1 antibody 68304-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols