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ATP5F1 Monoclonal antibody, PBS Only

ATP5F1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A10G9

Cat no : 68304-1-PBS

Print datasheet

Synonyms

ATP5F1, ATPase subunit b, PIG47

验证数据展示

  • WB analysis using 68304-1-Ig (same clone as 68304-1-PBS)

    WB analysis using 68304-1-Ig (same clone as 68304-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68304-1-Ig (ATP5F1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68304-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 68304-1-Ig (same clone as 68304-1-PBS)

    IF Staining of HeLa using 68304-1-Ig (same clone as 68304-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ATP5F1 antibody (68304-1-Ig, Clone: 1A10G9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68304-1-PBS in a different storage buffer formulation.

产品信息

68304-1-PBS targets ATP5F1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen ATP5F1 fusion protein Ag8571 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ATP synthase, H+ transporting, mitochondrial F0 complex, subunit B1
Synonyms ATP5F1, ATPase subunit b, PIG47
Calculated Molecular Weight 256 aa, 29 kDa
Observed Molecular Weight 25-30 kDa
GenBank Accession NumberBC005366
Gene Symbol ATP5F1
Gene ID (NCBI) 515
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP24539
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ATP5F1(ATP synthase subunit b) belongs to the eukaryotic ATPase B chain family. The ATP5F1 gene encodes subunit B of the mitochondrial ATP synthase Fo unit, which contains 214-amino acid with a 42-amino acid import signal(PMID:1831354). Mitochondrial membrane ATP synthase(F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain.