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ATP5F1 Monoclonal antibody, PBS Only

ATP5F1 Monoclonal Antibody for WB,Indirect ELISA,IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB,Indirect ELISA,IF

Conjugate

Unconjugated

CloneNo.

1A10G9

Cat No : 68304-1-PBS

Print datasheet

Synonyms

ATP5F1, ATPase subunit b, PIG47

验证数据展示

  • WB analysis using 68304-1-Ig (same clone as 68304-1-PBS)

    WB analysis using 68304-1-Ig (same clone as 68304-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68304-1-Ig (ATP5F1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68304-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 68304-1-Ig (same clone as 68304-1-PBS)

    IF Staining of HeLa using 68304-1-Ig (same clone as 68304-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ATP5F1 antibody (68304-1-Ig, Clone: 1A10G9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68304-1-PBS in a different storage buffer formulation.

产品信息

68304-1-PBS targets ATP5F1 in WB, IF, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB,Indirect ELISA,IF Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen ATP5F1 fusion protein Ag8571 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ATP synthase, H+ transporting, mitochondrial F0 complex, subunit B1
Synonyms ATP5F1, ATPase subunit b, PIG47
Calculated Molecular Weight 256 aa, 29 kDa
Observed Molecular Weight 25-30 kDa
GenBank Accession NumberBC005366
Gene Symbol ATP5F1
Gene ID (NCBI) 515
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP24539
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

ATP5F1(ATP synthase subunit b) belongs to the eukaryotic ATPase B chain family. The ATP5F1 gene encodes subunit B of the mitochondrial ATP synthase Fo unit, which contains 214-amino acid with a 42-amino acid import signal(PMID:1831354). Mitochondrial membrane ATP synthase(F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain.