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验证数据展示

  • WB analysis using 68306-1-Ig

    WB analysis using 68306-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68306-1-Ig (DHX32 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 68306-1-Ig

    WB analysis of A549 using 68306-1-Ig

    WB result of DHX32 antibody (68306-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DHX32 transfected A549 cells.

  • FC experiment of HeLa using 68306-1-Ig

    FC experiment of HeLa using 68306-1-Ig

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug DHX32 Monoclonal antibody (68306-1-Ig, Clone:2A4G9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO验证

DHX32 Monoclonal antibody

DHX32 Monoclonal Antibody for WB, ELISA, FC (Intra)
Cat No. 68306-1-Ig

产品说明书

CloneNo. 2A4G9

宿主/亚型

Mouse / IgG2b

种属反应性

Human, Mouse, Rat

应用

WB, ELISA, FC (Intra)

别名

HuDDX32, EC:3.6.4.13, DHLP1, DEAD/H helicase-like protein 1, DDX32

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, U2OS cells, rat testis tissue, HeLa cells, A549 cells, mouse testis tissue, MCF-7 cells, K-562 cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68306-1-Ig targets DHX32 in WB, FC (Intra), ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, ELISA, FC (Intra) Application Description
经测试反应性 Human, Mouse, Rat
免疫原 DHX32 fusion protein Ag13956 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 DEAH (Asp-Glu-Ala-His) box polypeptide 32
别名 HuDDX32, EC:3.6.4.13, DHLP1, DEAD/H helicase-like protein 1, DDX32
计算分子量 743 aa, 84 kDa
观测分子量 84 kDa
GenBank蛋白编号BC002473
基因名称 DHX32
Gene ID (NCBI) 55760
RRIDAB_2935385
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ7L7V1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DHX32, an RNA helicase, belongs to the DEAH box RNA helicase family by virtue of its homology to the conserved helicase domain. DHX32 is abundantly expressed in human and murine models, participating in cell transcription and differentiation, and being capable of regulating T-cell apoptosis. DHX32 is also associated with some malignant tumors, such as lymphoma, colorectal cancer, and breast cancer (PMID: 35117530). DHX32 has 2 isoforms with molecular weights of 75 and 84 kDa, respectively.

实验方案

Product Specific Protocols
WB protocol for DHX32 antibody 68306-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols