验证数据展示
经过测试的应用
| Positive WB detected in | U2OS cells, Saos-2 cells, HeLa cells, HEK-293 cells, MDA-MB-231 cells, Jurkat cells, K-562 cells, HSC-T6 cells, 4T1 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68313-1-Ig targets GTSE1 in WB, ELISA applications and shows reactivity with Human, Rat, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human, Rat, mouse |
| 免疫原 | GTSE1 fusion protein Ag32817 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | G-2 and S-phase expressed 1 |
| 别名 | B99, G 2 and S phase expressed 1, GTSE 1, GTSE1, Protein B99 homolog |
| 计算分子量 | 77 kDa |
| 观测分子量 | 67 kDa |
| GenBank蛋白编号 | BC006325 |
| 基因名称 | GTSE1 |
| Gene ID (NCBI) | 51512 |
| RRID | AB_2935391 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q9NYZ3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GTSE1 is only expressed in the S and G2 phases of the cell cycle, where it colocalizes with cytoplasmic tubulin and microtubules. In response to DNA damage, the encoded protein accumulates in the nucleus and binds the tumor suppressor protein p53, shutting it out of the nucleus and repressing its ability to induce apoptosis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GTSE1 antibody 68313-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |