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NDUFS6 Monoclonal antibody

NDUFS6 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Rabbit, Chicken

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2F4H3

Cat no : 68329-1-Ig

Print datasheet

Synonyms

CI 13kD A, Complex I 13kD A, NDUFS6

验证数据展示

  • WB analysis of rabbit heart using 68329-1-Ig

    WB analysis of rabbit heart using 68329-1-Ig

    rabbit heart tissue were subjected to SDS PAGE followed by western blot with 68329-1-Ig (NDUFS6 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 68329-1-Ig

    IHC staining of human prostate cancer using 68329-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 68329-1-Ig (NDUFS6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 68329-1-Ig

    IHC staining of human prostate cancer using 68329-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 68329-1-Ig (NDUFS6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 68329-1-Ig

    IF Staining of HepG2 using 68329-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NDUFS6 antibody (68329-1-Ig, Clone: 2F4H3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

  • WB analysis of HepG2 using 68329-1-Ig

    WB analysis of HepG2 using 68329-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 68329-1-Ig (NDUFS6 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inrabbit heart tissue, HepG2 cells, rat heart tissue, mouse heart tissue, chicken heart tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68329-1-Ig targets NDUFS6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Chicken samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity Human, Mouse, Rat, Rabbit, Chicken
Immunogen NDUFS6 fusion protein Ag6286 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name NADH dehydrogenase (ubiquinone) Fe-S protein 6, 13kDa (NADH-coenzyme Q reductase)
Synonyms CI 13kD A, Complex I 13kD A, NDUFS6
Calculated Molecular Weight 14 kDa
Observed Molecular Weight13-15 kDa
GenBank Accession NumberBC046155
Gene Symbol NDUFS6
Gene ID (NCBI) 4726
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO75380
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NDUFS6(NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial) is also named as CI-13kD-A, NADH-ubiquinone oxidoreductase 13 kDa-A subunit and belongs to the complex I NDUFS6 subunit family.It is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis.

实验方案

Product Specific Protocols
WB protocol for NDUFS6 antibody 68329-1-IgDownload protocol
IHC protocol for NDUFS6 antibody 68329-1-IgDownload protocol
IF protocol for NDUFS6 antibody 68329-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols