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ALAS2 Monoclonal antibody

ALAS2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2C1H1

Cat no : 68332-1-Ig

Print datasheet

Synonyms

ALAS E, ALAS2, ALASE, ANH1, ASB, Delta ALA synthase 2, FLJ93603, XLSA

验证数据展示

  • WB analysis using 68332-1-Ig

    WB analysis using 68332-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68332-1-Ig (ALAS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68332-1-Ig

    WB analysis using 68332-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68332-1-Ig (ALAS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68332-1-Ig

    WB analysis using 68332-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68332-1-Ig (ALAS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of NK-92 using 68332-1-Ig

    WB analysis of NK-92 using 68332-1-Ig

    NK-92 cells were subjected to SDS PAGE followed by western blot with 68332-1-Ig (ALAS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inTHP-1 cells, HEK-293 cells, Daudi cells, NK-92 cells, Raji cells, MOLT-4 cells, K-562 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68332-1-Ig targets ALAS2 in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen ALAS2 fusion protein Ag13524 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name aminolevulinate, delta-, synthase 2
Synonyms ALAS E, ALAS2, ALASE, ANH1, ASB, Delta ALA synthase 2, FLJ93603, XLSA
Calculated Molecular Weight 587 aa, 65 kDa
Observed Molecular Weight60~65 kDa
GenBank Accession NumberBC030230
Gene Symbol ALAS2
Gene ID (NCBI) 212
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP22557
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The heme biosynthetic pathway begins from delta aminolevulinic acid synthase (ALAS) catalyzing the condensation of glycine and succinyl-CoA to delta aminolevulinic acid (ALA) in the mitochondria. ALAS is coded by two genes: ALAS1 and ALAS2 . ALAS1 is ubiquitously expressed in all cells, and the negative feedback is regulated by the heme pool; however, ALAS2 is specifically expressed only in erythroid cells.

实验方案

Product Specific Protocols
WB protocol for ALAS2 antibody 68332-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols