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验证数据展示

  • WB analysis using 68332-1-Ig

    WB analysis using 68332-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68332-1-Ig (ALAS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68332-1-Ig

    WB analysis using 68332-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68332-1-Ig (ALAS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68332-1-Ig

    WB analysis using 68332-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68332-1-Ig (ALAS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of NK-92 using 68332-1-Ig

    WB analysis of NK-92 using 68332-1-Ig

    NK-92 cells were subjected to SDS PAGE followed by western blot with 68332-1-Ig (ALAS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

ALAS2 Monoclonal antibody

ALAS2 Monoclonal Antibody for WB, ELISA
Cat No. 68332-1-Ig

产品说明书

CloneNo. 2C1H1

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, ELISA

别名

ALAS E, ALAS2, ALASE, ANH1, ASB, Delta ALA synthase 2, FLJ93603, XLSA

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inTHP-1 cells, HEK-293 cells, Daudi cells, NK-92 cells, Raji cells, MOLT-4 cells, K-562 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68332-1-Ig targets ALAS2 in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原

CatNo: Ag13524

Product name: Recombinant human ALAS2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 224-574 aa of BC030230

Sequence: VELEQELAELHQKDSALLFSSCFVANDSTLFTLAKILPGCEIYSDAGNHASMIQGIRNSGAAKFVFRHNDPDHLKKLLEKSNPKIPKIVAFETVHSMDGAICPLEELCDVSHQYGALTFVDEVHAVGLYGSRGAGIGERDGIMHKIDIISGTLGKAFGCVGGYIASTRDLVDMVRSYAAGFIFTTSLPPMVLSGALESVRLLKGEEGQALRRAHQRNVKHMRQLLMDRGLPVIPCPSHIIPIRVGNAALNSKLCDLLLSKHGIYVQAINYPTVPRGEELLRLAPSPHHSPQMMEDFVEKLLLAWTAVGLPLQDVSVAACNFCRRPVHFELMSEWERSYFGNMGPQYVTTYA

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 aminolevulinate, delta-, synthase 2
别名 ALAS E, ALAS2, ALASE, ANH1, ASB, Delta ALA synthase 2, FLJ93603, XLSA
计算分子量 587 aa, 65 kDa
观测分子量60~65 kDa
GenBank蛋白编号BC030230
基因名称 ALAS2
Gene ID (NCBI) 212
RRIDAB_3085057
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP22557
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The heme biosynthetic pathway begins from delta aminolevulinic acid synthase (ALAS) catalyzing the condensation of glycine and succinyl-CoA to delta aminolevulinic acid (ALA) in the mitochondria. ALAS is coded by two genes: ALAS1 and ALAS2 . ALAS1 is ubiquitously expressed in all cells, and the negative feedback is regulated by the heme pool; however, ALAS2 is specifically expressed only in erythroid cells.

实验方案

Product Specific Protocols
WB protocol for ALAS2 antibody 68332-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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