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验证数据展示

  • WB analysis using 68332-1-Ig (same clone as 68332-1-PBS)

    WB analysis using 68332-1-Ig (same clone as 68332-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68332-1-Ig (ALAS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68332-1-PBS in a different storage buffer formulation.

  • WB analysis using 68332-1-Ig (same clone as 68332-1-PBS)

    WB analysis using 68332-1-Ig (same clone as 68332-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68332-1-Ig (ALAS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68332-1-PBS in a different storage buffer formulation.

  • WB analysis using 68332-1-Ig (same clone as 68332-1-PBS)

    WB analysis using 68332-1-Ig (same clone as 68332-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68332-1-Ig (ALAS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68332-1-PBS in a different storage buffer formulation.

  • WB analysis of NK-92 using 68332-1-Ig (same clone as 68332-1-PBS)

    WB analysis of NK-92 using 68332-1-Ig (same clone as 68332-1-PBS)

    NK-92 cells were subjected to SDS PAGE followed by western blot with 68332-1-Ig (ALAS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68332-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50624-1

    Cytometric bead array standard curve of MP50624-1

    Cytometric bead array standard curve of MP50624-1, ALAS2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68332-1-PBS. Detection antibody: 68332-2-PBS. Standard:Ag13524. Range: 6.25-100 ng/mL.

  • Cytometric bead array standard curve of MP50624-2

    Cytometric bead array standard curve of MP50624-2

    Cytometric bead array standard curve of MP50624-2, ALAS2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68332-1-PBS. Detection antibody: 68332-3-PBS. Standard:Ag13524. Range: 3.125-100 ng/mL.

ALAS2 Monoclonal antibody, PBS Only (Capture)

ALAS2 Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA
Cat No. 68332-1-PBS

产品说明书

CloneNo. 2C1H1

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, Cytometric bead array, Indirect ELISA

别名

ALASE, ALAS E, ALAS 2, 5-aminolevulinic acid synthase 2, 5-aminolevulinate synthase, erythroid-specific, mitochondrial

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

68332-1-PBS targets ALAS2 as part of a matched antibody pair:

MP50624-1: 68332-1-PBS capture and 68332-2-PBS detection (validated in Cytometric bead array)

MP50624-2: 68332-1-PBS capture and 68332-3-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 ALAS2 fusion protein Ag13524 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 aminolevulinate, delta-, synthase 2
别名 ALASE, ALAS E, ALAS 2, 5-aminolevulinic acid synthase 2, 5-aminolevulinate synthase, erythroid-specific, mitochondrial
计算分子量 587 aa, 65 kDa
观测分子量60~65 kDa
GenBank蛋白编号BC030230
基因名称 ALAS2
Gene ID (NCBI) 212
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP22557
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The heme biosynthetic pathway begins from delta aminolevulinic acid synthase (ALAS) catalyzing the condensation of glycine and succinyl-CoA to delta aminolevulinic acid (ALA) in the mitochondria. ALAS is coded by two genes: ALAS1 and ALAS2 . ALAS1 is ubiquitously expressed in all cells, and the negative feedback is regulated by the heme pool; however, ALAS2 is specifically expressed only in erythroid cells.