验证数据展示
经过测试的应用
| Positive WB detected in | THP-1 cells, HEK-293 cells, Daudi cells, NK-92 cells, Raji cells, MOLT-4 cells, K-562 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68332-1-Ig targets ALAS2 in WB, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human |
| 免疫原 | ALAS2 fusion protein Ag13524 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | aminolevulinate, delta-, synthase 2 |
| 别名 | ALAS E, ALAS2, ALASE, ANH1, ASB, Delta ALA synthase 2, FLJ93603, XLSA |
| 计算分子量 | 587 aa, 65 kDa |
| 观测分子量 | 60~65 kDa |
| GenBank蛋白编号 | BC030230 |
| 基因名称 | ALAS2 |
| Gene ID (NCBI) | 212 |
| RRID | AB_3085057 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P22557 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The heme biosynthetic pathway begins from delta aminolevulinic acid synthase (ALAS) catalyzing the condensation of glycine and succinyl-CoA to delta aminolevulinic acid (ALA) in the mitochondria. ALAS is coded by two genes: ALAS1 and ALAS2 . ALAS1 is ubiquitously expressed in all cells, and the negative feedback is regulated by the heme pool; however, ALAS2 is specifically expressed only in erythroid cells.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ALAS2 antibody 68332-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |