验证数据展示
经过测试的应用
| Positive WB detected in | fetal human brain tissue, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue, pig cerebellum tissue, rabbit cerebellum tissue, rat cerebellum tissue, mouse cerebellum tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68340-1-Ig targets GDAP1L1 in WB, ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human, mouse, rat, pig, rabbit |
| 免疫原 | GDAP1L1 fusion protein Ag27717 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | ganglioside-induced differentiation-associated protein 1-like 1 |
| 别名 | dJ881L22.1, dJ995J12.1.1, DKFZp761K228, GDAP1 L1, GDAP1L1 |
| 计算分子量 | 367 aa, 42 kDa |
| 观测分子量 | 38-40 kDa |
| GenBank蛋白编号 | BC009014 |
| 基因名称 | GDAP1L1 |
| Gene ID (NCBI) | 78997 |
| RRID | AB_3085064 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q96MZ0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ganglioside-induced differentiation-associated protein 1-like 1 (GDAP1L1) belongs to GST superfamily. Owing to its central role in mitochondrial fission, GDAP1L1 is a homologue of GDAP1 implicated in cellular processes, such as cell death, autophagy, aging, and immune response regulation (PMID:32230997). GDAP1L1, a fission factor controlling mitochondrial fission, induces fission upon translocation from the cytosol to mitochondria in response to stress condition (PMID:34638757). Furthermore, the activity of GDAP1L1 is dependent on Drp1. GDAP1, a paralog of GDAP1L1, evokes mitochondrial fragmentation only in the presence of Drp1 in HeLa cells (PMID:19782751).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GDAP1L1 antibody 68340-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |