IRSp53 Monoclonal antibody
IRSp53 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse, Rat, Rabbit
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
1D4G1
验证数据展示
经过测试的应用
| Positive WB detected in | rabbit brain tissue, LNCaP cells, rabbit cerebellum tissue, rat brain tissue, mouse brain tissue |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68348-1-Ig targets IRSp53 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit samples.
| Tested Applications | WB, ELISA Application Description |
| Tested Reactivity | Human, Mouse, Rat, Rabbit |
| Immunogen | IRSp53 fusion protein Ag25161 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | BAI1-associated protein 2 |
| Synonyms | BAI associated protein 2, BAI1 associated protein 2, BAIAP2, BAP2, Fas ligand associated factor 3, FLAF3, Insulin receptor substrate p53, IRS 58, IRSP53, IRSp53/58, Protein BAP2 |
| Calculated Molecular Weight | 61 kDa |
| Observed Molecular Weight | 53 kDa |
| GenBank Accession Number | BC014020 |
| Gene Symbol | IRSp53 |
| Gene ID (NCBI) | 10458 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9UQB8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IRSp53 (also known as BAIAP2) is a multi-domain adaptor protein that originally identified as a 58/53-kDa substrate of insulin-receptor kinase in the hamster (PMID: 10332026). As a BAI1-binding protein, IRSp53 plays an important role in linking between membrane and cytoskeleton in the process of neuronal growth (PMID: 10343108). IRSp53 is necessary for Cdc42-mediated reorganization of the actin cytoskeleton and for Rac1-mediated membrane ruffling. Alternatively spliced isoforms of IRSp53 exist. IRSp53 has been implicated in several psychiatric disorders, including autism spectrum disorders (ASDs), schizophrenia, and attention deficit/hyperactivity disorder (ADHD) (PMID: 26275848).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for IRSp53 antibody 68348-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
