• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 68357-1-Ig

    WB analysis using 68357-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68357-1-Ig (DCTD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IF Staining of HepG2 using 68357-1-Ig

    IF Staining of HepG2 using 68357-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DCTD antibody (68357-1-Ig, Clone: 3D2G12 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

DCTD Monoclonal antibody

DCTD Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68357-1-Ig

产品说明书

CloneNo. 3D2G12
引用文献(1)

宿主/亚型

Mouse / IgG1

种属反应性

Human and More (1)

应用

WB, IF/ICC, ELISA

别名

dCMP deaminase, DCTD, Deoxycytidylate deaminase

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inA549 cells, U2OS cells, HeLa cells, LNCaP cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

68357-1-Ig targets DCTD in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 Human
文献引用反应性human
免疫原 DCTD fusion protein Ag10245 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 dCMP deaminase
别名 dCMP deaminase, DCTD, Deoxycytidylate deaminase
计算分子量 178 aa, 20 kDa
观测分子量18-20 kDa
GenBank蛋白编号BC001286
基因名称 DCTD
Gene ID (NCBI) 1635
RRIDAB_3085079
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP32321
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DCTD(Deoxycytidylate deaminase) is also named as dCMP deaminase and belongs to the cytidine and deoxycytidylate deaminase family. It catalyzes the deamination of dCMP to dUMP, thus providing the nucleotide substrate for thymidylate synthase. Control of deaminase activity at this juncture in deoxyribonucleotide metabolism is determined by the ratio of dCTP to dTTP in the cell, since the enzyme is allosterically activated by dCTP and inhibited by dTTP(PMID:7685356). It has 2 isoforms produced by alternative splicing and can exsit as a dimer(pMID:8798492).

实验方案

Product Specific Protocols
WB protocol for DCTD antibody 68357-1-IgDownload protocol
IF protocol for DCTD antibody 68357-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Molecules

Teadenol B as a Component of Microorganism-Fermented Tea Extract Inhibited Breast Cancers by Promoting Autophagy

Authors - Ying Zhao
View Article