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DCTD Monoclonal antibody, PBS Only

DCTD Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3D2G12

Cat No : 68357-1-PBS

Print datasheet

Synonyms

dCMP deaminase, DCTD, Deoxycytidylate deaminase

验证数据展示

  • WB analysis using 68357-1-Ig (same clone as 68357-1-PBS)

    WB analysis using 68357-1-Ig (same clone as 68357-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68357-1-Ig (DCTD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68357-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 68357-1-Ig (same clone as 68357-1-PBS)

    IF Staining of HepG2 using 68357-1-Ig (same clone as 68357-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DCTD antibody (68357-1-Ig, Clone: 3D2G12 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68357-1-PBS in a different storage buffer formulation.

产品信息

68357-1-PBS targets DCTD in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen DCTD fusion protein Ag10245 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name dCMP deaminase
Synonyms dCMP deaminase, DCTD, Deoxycytidylate deaminase
Calculated Molecular Weight 178 aa, 20 kDa
Observed Molecular Weight18-20 kDa
GenBank Accession NumberBC001286
Gene Symbol DCTD
Gene ID (NCBI) 1635
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP32321
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DCTD(Deoxycytidylate deaminase) is also named as dCMP deaminase and belongs to the cytidine and deoxycytidylate deaminase family. It catalyzes the deamination of dCMP to dUMP, thus providing the nucleotide substrate for thymidylate synthase. Control of deaminase activity at this juncture in deoxyribonucleotide metabolism is determined by the ratio of dCTP to dTTP in the cell, since the enzyme is allosterically activated by dCTP and inhibited by dTTP(PMID:7685356). It has 2 isoforms produced by alternative splicing and can exsit as a dimer(pMID:8798492).