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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 68357-1-Ig (same clone as 68357-1-PBS)

    WB analysis using 68357-1-Ig (same clone as 68357-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68357-1-Ig (DCTD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68357-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 68357-1-Ig (same clone as 68357-1-PBS)

    IF Staining of HepG2 using 68357-1-Ig (same clone as 68357-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DCTD antibody (68357-1-Ig, Clone: 3D2G12 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68357-1-PBS in a different storage buffer formulation.

DCTD Monoclonal antibody, PBS Only

DCTD Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68357-1-PBS

产品说明书

CloneNo. 3D2G12

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, IF/ICC, Indirect ELISA

别名

dCMP deaminase, DCTD, Deoxycytidylate deaminase

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

68357-1-PBS targets DCTD in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human
免疫原 DCTD fusion protein Ag10245 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 dCMP deaminase
别名 dCMP deaminase, DCTD, Deoxycytidylate deaminase
计算分子量 178 aa, 20 kDa
观测分子量18-20 kDa
GenBank蛋白编号BC001286
基因名称 DCTD
Gene ID (NCBI) 1635
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP32321
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DCTD(Deoxycytidylate deaminase) is also named as dCMP deaminase and belongs to the cytidine and deoxycytidylate deaminase family. It catalyzes the deamination of dCMP to dUMP, thus providing the nucleotide substrate for thymidylate synthase. Control of deaminase activity at this juncture in deoxyribonucleotide metabolism is determined by the ratio of dCTP to dTTP in the cell, since the enzyme is allosterically activated by dCTP and inhibited by dTTP(PMID:7685356). It has 2 isoforms produced by alternative splicing and can exsit as a dimer(pMID:8798492).