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DCTD Monoclonal antibody

DCTD Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3D2G12

Cat no : 68357-1-Ig

Print datasheet

Synonyms

dCMP deaminase, DCTD, Deoxycytidylate deaminase

验证数据展示

  • WB analysis using 68357-1-Ig

    WB analysis using 68357-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68357-1-Ig (DCTD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IF Staining of HepG2 using 68357-1-Ig

    IF Staining of HepG2 using 68357-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DCTD antibody (68357-1-Ig, Clone: 3D2G12 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inA549 cells, U2OS cells, HeLa cells, LNCaP cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

68357-1-Ig targets DCTD in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human
Cited Reactivityhuman
Immunogen DCTD fusion protein Ag10245 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name dCMP deaminase
Synonyms dCMP deaminase, DCTD, Deoxycytidylate deaminase
Calculated Molecular Weight 178 aa, 20 kDa
Observed Molecular Weight18-20 kDa
GenBank Accession NumberBC001286
Gene Symbol DCTD
Gene ID (NCBI) 1635
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP32321
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DCTD(Deoxycytidylate deaminase) is also named as dCMP deaminase and belongs to the cytidine and deoxycytidylate deaminase family. It catalyzes the deamination of dCMP to dUMP, thus providing the nucleotide substrate for thymidylate synthase. Control of deaminase activity at this juncture in deoxyribonucleotide metabolism is determined by the ratio of dCTP to dTTP in the cell, since the enzyme is allosterically activated by dCTP and inhibited by dTTP(PMID:7685356). It has 2 isoforms produced by alternative splicing and can exsit as a dimer(pMID:8798492).

实验方案

Product Specific Protocols
WB protocol for DCTD antibody 68357-1-IgDownload protocol
IF protocol for DCTD antibody 68357-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Molecules

Teadenol B as a Component of Microorganism-Fermented Tea Extract Inhibited Breast Cancers by Promoting Autophagy

Authors - Ying Zhao
View Article