验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, U2OS cells, HeLa cells, LNCaP cells, HEK-293 cells, Jurkat cells, K-562 cells |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
68357-1-Ig targets DCTD in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human |
| 文献引用反应性 | human |
| 免疫原 | DCTD fusion protein Ag10245 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | dCMP deaminase |
| 别名 | dCMP deaminase, DCTD, Deoxycytidylate deaminase |
| 计算分子量 | 178 aa, 20 kDa |
| 观测分子量 | 18-20 kDa |
| GenBank蛋白编号 | BC001286 |
| 基因名称 | DCTD |
| Gene ID (NCBI) | 1635 |
| RRID | AB_3085079 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P32321 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DCTD(Deoxycytidylate deaminase) is also named as dCMP deaminase and belongs to the cytidine and deoxycytidylate deaminase family. It catalyzes the deamination of dCMP to dUMP, thus providing the nucleotide substrate for thymidylate synthase. Control of deaminase activity at this juncture in deoxyribonucleotide metabolism is determined by the ratio of dCTP to dTTP in the cell, since the enzyme is allosterically activated by dCTP and inhibited by dTTP(PMID:7685356). It has 2 isoforms produced by alternative splicing and can exsit as a dimer(pMID:8798492).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DCTD antibody 68357-1-Ig | Download protocol |
| IF protocol for DCTD antibody 68357-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |