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验证数据展示

  • WB analysis of HCT 116 using 68379-1-Ig (same clone as 68379-1-PBS)

    WB analysis of HCT 116 using 68379-1-Ig (same clone as 68379-1-PBS)

    HCT 116 cells were subjected to SDS PAGE followed by western blot with 68379-1-Ig (CREB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68379-1-PBS in a different storage buffer formulation.

  • WB analysis using 68379-1-Ig (same clone as 68379-1-PBS)

    WB analysis using 68379-1-Ig (same clone as 68379-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68379-1-Ig (CREB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68379-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 68379-1-Ig (same clone as 68379-1-PBS)

    IF Staining of U2OS using 68379-1-Ig (same clone as 68379-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CREB3 antibody (68379-1-Ig, Clone: 1C12H11 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68379-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50828-4

    Cytometric bead array standard curve of MP50828-4

    Cytometric bead array standard curve of MP50828-4, CREB3 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68379-7-PBS. Detection antibody: 68379-1-PBS. Standard:Ag1823. Range: 1.563-100 ng/mL.

CREB3 Monoclonal antibody, PBS Only (Detector)

CREB3 Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 68379-1-PBS

产品说明书

CloneNo. 1C12H11

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

Leucine zipper protein, Cyclic AMP-responsive element-binding protein 3, CREB-3, CREB 3, cAMP-responsive element-binding protein 3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CREB3 Ready-To-Use IHC Kit. CREB3 primary antibody included.

产品信息

68379-1-PBS targets CREB3 as part of a matched antibody pair:

MP50828-4: 68379-7-PBS capture and 68379-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 CREB3 fusion protein Ag1823 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 cAMP responsive element binding protein 3
别名 Leucine zipper protein, Cyclic AMP-responsive element-binding protein 3, CREB-3, CREB 3, cAMP-responsive element-binding protein 3
计算分子量 44 kDa
观测分子量 40-64 kDa
GenBank蛋白编号BC009402
基因名称 CREB3
Gene ID (NCBI) 10488
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO43889
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CREB3, also named as LZIP and Luman, belongs to the bZIP family and ATF subfamily. It is a transcription factors activated upon intramembrane proteolysis (RIP), binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AG][AG]-3'), a sequence present in many viral and cellular promoters. CREB3 binds to and requires HCFC1 as a coactivator. The activity and expression are suppressed when the HCFC1-CREB3 complex binds with CREBZF. Participates in LKN-1/CCL15-induced chemotaxis signaling. CREB3 is N-glycosylated and released by proteolysis. So the MW of CREB3 emirate 40kd to 64kd (PMID: 12138176).