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验证数据展示

  • WB analysis of HCT 116 using 68379-1-Ig

    WB analysis of HCT 116 using 68379-1-Ig

    HCT 116 cells were subjected to SDS PAGE followed by western blot with 68379-1-Ig (CREB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68379-1-Ig

    WB analysis using 68379-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68379-1-Ig (CREB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 68379-1-Ig

    IF Staining of U2OS using 68379-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CREB3 antibody (68379-1-Ig, Clone: 1C12H11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

CREB3 Monoclonal antibody

CREB3 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68379-1-Ig

产品说明书

CloneNo. 1C12H11

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, IF/ICC, ELISA

别名

CREB 3, CREB3, LUMAN, LZIP

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, T-47D cells, HCT 116 cells, hTERT-RPE1 cells
Positive IF/ICC detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy CREB3 Ready-To-Use IHC Kit. CREB3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68379-1-Ig targets CREB3 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原 CREB3 fusion protein Ag1823 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 cAMP responsive element binding protein 3
别名 CREB 3, CREB3, LUMAN, LZIP
计算分子量 44 kDa
观测分子量 40-64 kDa
GenBank蛋白编号BC009402
基因名称 CREB3
Gene ID (NCBI) 10488
RRIDAB_3085097
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO43889
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CREB3, also named as LZIP and Luman, belongs to the bZIP family and ATF subfamily. It is a transcription factors activated upon intramembrane proteolysis (RIP), binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AG][AG]-3'), a sequence present in many viral and cellular promoters. CREB3 binds to and requires HCFC1 as a coactivator. The activity and expression are suppressed when the HCFC1-CREB3 complex binds with CREBZF. Participates in LKN-1/CCL15-induced chemotaxis signaling. CREB3 is N-glycosylated and released by proteolysis. So the MW of CREB3 emirate 40kd to 64kd (PMID: 12138176).

实验方案

Product Specific Protocols
WB protocol for CREB3 antibody 68379-1-IgDownload protocol
IF protocol for CREB3 antibody 68379-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols