• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CREB3 Monoclonal antibody, PBS Only

CREB3 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C12H11

Cat no : 68379-1-PBS

Print datasheet

Synonyms

CREB 3, CREB3, LUMAN, LZIP

验证数据展示

  • WB analysis of HCT 116 using 68379-1-Ig (same clone as 68379-1-PBS)

    WB analysis of HCT 116 using 68379-1-Ig (same clone as 68379-1-PBS)

    HCT 116 cells were subjected to SDS PAGE followed by western blot with 68379-1-Ig (CREB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68379-1-PBS in a different storage buffer formulation.

  • WB analysis using 68379-1-Ig (same clone as 68379-1-PBS)

    WB analysis using 68379-1-Ig (same clone as 68379-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68379-1-Ig (CREB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68379-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 68379-1-Ig (same clone as 68379-1-PBS)

    IF Staining of U2OS using 68379-1-Ig (same clone as 68379-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CREB3 antibody (68379-1-Ig, Clone: 1C12H11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68379-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CREB3 Ready-To-Use IHC Kit. CREB3 primary antibody included.

产品信息

68379-1-PBS targets CREB3 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen CREB3 fusion protein Ag1823 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name cAMP responsive element binding protein 3
Synonyms CREB 3, CREB3, LUMAN, LZIP
Calculated Molecular Weight 44 kDa
Observed Molecular Weight 40-64 kDa
GenBank Accession NumberBC009402
Gene Symbol CREB3
Gene ID (NCBI) 10488
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO43889
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CREB3, also named as LZIP and Luman, belongs to the bZIP family and ATF subfamily. It is a transcription factors activated upon intramembrane proteolysis (RIP), binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AG][AG]-3'), a sequence present in many viral and cellular promoters. CREB3 binds to and requires HCFC1 as a coactivator. The activity and expression are suppressed when the HCFC1-CREB3 complex binds with CREBZF. Participates in LKN-1/CCL15-induced chemotaxis signaling. CREB3 is N-glycosylated and released by proteolysis. So the MW of CREB3 emirate 40kd to 64kd (PMID: 12138176).