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验证数据展示

  • IF Staining of HeLa using 68419-1-Ig

    IF Staining of HeLa using 68419-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Hexokinase 1 antibody (68419-1-Ig, Clone: 2B4F9 ) at dilution of 1:2049 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004).

  • WB analysis using 68419-1-Ig

    WB analysis using 68419-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68419-1-Ig (HK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 68419-1-Ig

    IP experiment of HeLa using 68419-1-Ig

    IP result of anti-Hexokinase 1 (IP:68419-1-Ig, 4ug; Detection:68419-1-Ig 1:2000) with HeLa cells lysate 1560 ug.

  • IHC staining of mouse brain using 68419-1-Ig

    IHC staining of mouse brain using 68419-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68419-1-Ig (Hexokinase 1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Hexokinase 1 Monoclonal antibody

Hexokinase 1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 68419-1-Ig

产品说明书

CloneNo. 2B4F9

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, IP, ELISA

别名

HK1, Hexokinase-1, Hexokinase1, Hexokinase type I, EC:2.7.1.1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HT-1080 cells, U-87 MG cells, SK-BR-3 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, pig brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:1024-1:4098
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68419-1-Ig targets Hexokinase 1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 Hexokinase 1 fusion protein Ag8015 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 hexokinase 1
别名 HK1, Hexokinase-1, Hexokinase1, Hexokinase type I, EC:2.7.1.1
计算分子量 102 kDa
观测分子量100 kDa
GenBank蛋白编号BC008730
基因名称 Hexokinase 1
Gene ID (NCBI) 3098
RRIDAB_3085134
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP19367
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The hexokinases (HKs) catalyze the first obligatory step in glucose metabolism to generate glucose-6-phosphate. This not only furthers glucose entry by maintaining the concentration gradient for facilitated glucose influx but also provides the first intermediate for essentially all major pathways using glucose. There are four conventional HK isoforms, HK1/2/3/4, encoded by four different genes. Most adult tissues express only HK1. Muscle and adipose tissue use HK2 for glycolysis, liver, and pancreatic beta cells express HK4 (also called glucokinase) and do not express HK1 or HK2. (PMID: 31434645, PMID: 31848318)

实验方案

Product Specific Protocols
WB protocol for Hexokinase 1 antibody 68419-1-IgDownload protocol
IHC protocol for Hexokinase 1 antibody 68419-1-IgDownload protocol
IF protocol for Hexokinase 1 antibody 68419-1-IgDownload protocol
IP protocol for Hexokinase 1 antibody 68419-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols