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验证数据展示

  • IF Staining of HeLa using 68419-1-Ig (same clone as 68419-1-PBS)

    IF Staining of HeLa using 68419-1-Ig (same clone as 68419-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Hexokinase 1 antibody (68419-1-Ig, Clone: 2B4F9 ) at dilution of 1:2049 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004). This data was developed using the same antibody clone with 68419-1-PBS in a different storage buffer formulation.

  • WB analysis using 68419-1-Ig (same clone as 68419-1-PBS)

    WB analysis using 68419-1-Ig (same clone as 68419-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68419-1-Ig (HK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68419-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 68419-1-Ig (same clone as 68419-1-PBS)

    IP experiment of HeLa using 68419-1-Ig (same clone as 68419-1-PBS)

    IP result of anti-Hexokinase 1 (IP:68419-1-Ig, 4ug; Detection:68419-1-Ig 1:2000) with HeLa cells lysate 1560 ug. This data was developed using the same antibody clone with 68419-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 68419-1-Ig (same clone as 68419-1-PBS)

    IHC staining of mouse brain using 68419-1-Ig (same clone as 68419-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68419-1-Ig (Hexokinase 1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68419-1-PBS in a different storage buffer formulation.

Hexokinase 1 Monoclonal antibody, PBS Only

Hexokinase 1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 68419-1-PBS

产品说明书

CloneNo. 2B4F9

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

HK1, Hexokinase-1, Hexokinase1, Hexokinase type I, EC:2.7.1.1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

68419-1-PBS targets Hexokinase 1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 Hexokinase 1 fusion protein Ag8015 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 hexokinase 1
别名 HK1, Hexokinase-1, Hexokinase1, Hexokinase type I, EC:2.7.1.1
计算分子量 102 kDa
观测分子量100 kDa
GenBank蛋白编号BC008730
基因名称 Hexokinase 1
Gene ID (NCBI) 3098
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP19367
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The hexokinases (HKs) catalyze the first obligatory step in glucose metabolism to generate glucose-6-phosphate. This not only furthers glucose entry by maintaining the concentration gradient for facilitated glucose influx but also provides the first intermediate for essentially all major pathways using glucose. There are four conventional HK isoforms, HK1/2/3/4, encoded by four different genes. Most adult tissues express only HK1. Muscle and adipose tissue use HK2 for glycolysis, liver, and pancreatic beta cells express HK4 (also called glucokinase) and do not express HK1 or HK2. (PMID: 31434645, PMID: 31848318)