Hexokinase 1 Monoclonal antibody
Hexokinase 1 Monoclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human, mouse, rat, pig
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
2B4F9
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HT-1080 cells, U-87 MG cells, SK-BR-3 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, pig brain tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68419-1-Ig targets Hexokinase 1 in WB, IP, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.
| Tested Applications | WB, IP, IHC, ELISA Application Description |
| Tested Reactivity | Human, mouse, rat, pig |
| Immunogen | Hexokinase 1 fusion protein Ag8015 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | hexokinase 1 |
| Synonyms | Brain form hexokinase, hexokinase 1, Hexokinase type I, HK I, HK1, HK1 ta, HK1 tb, HK1 tc, HKI, HXK1 |
| Calculated Molecular Weight | 102 kDa |
| Observed Molecular Weight | 100 kDa |
| GenBank Accession Number | BC008730 |
| Gene Symbol | Hexokinase 1 |
| Gene ID (NCBI) | 3098 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P19367 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The hexokinases (HKs) catalyze the first obligatory step in glucose metabolism to generate glucose-6-phosphate. This not only furthers glucose entry by maintaining the concentration gradient for facilitated glucose influx but also provides the first intermediate for essentially all major pathways using glucose. There are four conventional HK isoforms, HK1/2/3/4, encoded by four different genes. Most adult tissues express only HK1. Muscle and adipose tissue use HK2 for glycolysis, liver, and pancreatic beta cells express HK4 (also called glucokinase) and do not express HK1 or HK2. (PMID: 31434645, PMID: 31848318)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Hexokinase 1 antibody 68419-1-Ig | Download protocol |
| IHC protocol for Hexokinase 1 antibody 68419-1-Ig | Download protocol |
| IP protocol for Hexokinase 1 antibody 68419-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |