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验证数据展示

  • WB analysis using 68435-1-Ig

    WB analysis using 68435-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68435-1-Ig (BHLHE40 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

BHLHE40 Monoclonal antibody

BHLHE40 Monoclonal Antibody for WB, ELISA
Cat No. 68435-1-Ig

产品说明书

CloneNo. 1A3E1

宿主/亚型

Mouse / IgG2a

种属反应性

Human

应用

WB, ELISA

别名

BHLHB2, BHLHE40, DEC1, FLJ99214, SHARP 2, SHARP2, STRA13, Stra14

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inCalu-3 cells, A549 cells, HCT 116 cells, MDA-MB-468 cells, Saos-2 cells, HeLa cells, PC-3 cells, K-562 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68435-1-Ig targets BHLHE40 in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原 BHLHE40 fusion protein Ag12236 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 basic helix-loop-helix family, member e40
别名 BHLHB2, BHLHE40, DEC1, FLJ99214, SHARP 2, SHARP2, STRA13, Stra14
计算分子量 412 aa, 46 kDa
观测分子量 46-50 kDa
GenBank蛋白编号BC082238
基因名称 BHLHE40
Gene ID (NCBI) 8553
RRIDAB_3085148
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO14503
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BHLHE40 (Basic Helix-Loop-Helix Family Member E40), also known as BHLHB2, STRA13, DEC1, or SHARP2, is a member of the basic helix-loop-helix (bHLH) protein family, a large superfamily of transcriptional regulators expressed in many organisms. BHLHE40 is known to regulate a wide variety of essential cellular processes, including cell cycle, cellular proliferation, programmed cell death, cellular development and differentiation, as well as circadian rhythms (PMID: 34551158). It is reported that BHLHE40 is overexpressed in gastric, breast, and brain tumors; and downregulated in colorectal, esophageal, pancreatic and lung cancer (PMID: 32577154).

实验方案

Product Specific Protocols
WB protocol for BHLHE40 antibody 68435-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols