验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68436-1-Ig targets CNOT3 in WB, ELISA applications and shows reactivity with Human, pig , mouse, rat , rabbit samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human, pig , mouse, rat , rabbit |
| 免疫原 | CNOT3 fusion protein Ag33646 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | CCR4-NOT transcription complex, subunit 3 |
| 别名 | CCR4 associated factor 3, CNOT3, KIAA0691, LENG2, NOT3, NOT3H |
| 计算分子量 | 120 kDa |
| 观测分子量 | 120 kDa |
| GenBank蛋白编号 | BC016474 |
| 基因名称 | CNOT3 |
| Gene ID (NCBI) | 4849 |
| RRID | AB_3085149 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | O75175 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The CCR4-NOT complex, the major deadenylase in mammalian cells, shortens the mRNA poly(A) tail and leads to the destabilization of mRNAs. The CCR4-NOT complex plays pivotal roles in various physiological functions, including cell apoptosis, proliferation, and metabolism [PMID:22342980]. CNOT3, belongs to the CCR4-NOT complex, has a critical role in maintaining mouse and human ESC identity as a protein complex and inhibit differentiation into the extraembryonic lineages. It is also involved in the regulation of the spindle assembly checkpoint [PMID:22342980,22367759].
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CNOT3 antibody 68436-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |