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验证数据展示

  • WB analysis using 68450-1-Ig (same clone as 68450-1-PBS)

    WB analysis using 68450-1-Ig (same clone as 68450-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68450-1-Ig (OTUD6B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68450-1-PBS in a different storage buffer formulation.

  • IF Staining of PC-3 using 68450-1-Ig (same clone as 68450-1-PBS)

    IF Staining of PC-3 using 68450-1-Ig (same clone as 68450-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using OTUD6B antibody (68450-1-Ig, Clone: 1D1G1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68450-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50962-1

    Cytometric bead array standard curve of MP50962-1

    Cytometric bead array standard curve of MP50962-1, OTUD6B Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68450-1-PBS. Detection antibody: 68450-2-PBS. Standard:Ag33607. Range: 0.098-50 ng/mL.

  • Cytometric bead array standard curve of MP50962-2

    Cytometric bead array standard curve of MP50962-2

    Cytometric bead array standard curve of MP50962-2, OTUD6B Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68450-1-PBS. Detection antibody: 68450-3-PBS. Standard:Ag33607. Range: 0.098-25 ng/mL.

  • Cytometric bead array standard curve of MP50962-3

    Cytometric bead array standard curve of MP50962-3

    Cytometric bead array standard curve of MP50962-3, OTUD6B Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68450-1-PBS. Detection antibody: 68450-4-PBS. Standard:Ag33607. Range: 0.098-12.5 ng/mL.

OTUD6B Monoclonal antibody, PBS Only (Capture)

OTUD6B Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 68450-1-PBS

产品说明书

CloneNo. 1D1G1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

DUBA5, DUBA 5, Deubiquitinase OTUD6B, CGI-77, CGI 77

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

68450-1-PBS targets OTUD6B as part of a matched antibody pair:

MP50962-1: 68450-1-PBS capture and 68450-2-PBS detection (validated in Cytometric bead array)

MP50962-2: 68450-1-PBS capture and 68450-3-PBS detection (validated in Cytometric bead array)

MP50962-3: 68450-1-PBS capture and 68450-4-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 OTUD6B fusion protein Ag33607 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 OTU domain containing 6B
别名 DUBA5, DUBA 5, Deubiquitinase OTUD6B, CGI-77, CGI 77
计算分子量 293 aa, 34 kDa
观测分子量 34-40 kDa
GenBank蛋白编号BC029760
基因名称 OTUD6B
Gene ID (NCBI) 51633
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8N6M0
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

OTUD6B, also known as DUBA5, belongs to a DUB subfamily characterized by an ovarian tumor domain (OTU). It's a functional deubiquitinating enzyme, a class of protease that specifically cleaves ubiquitin linkages. OTUD6B function may be connected to growth and proliferation. It has been reported that OTUD6B could significantly suppress HCC cells migration and metastasis. The calculated molecular weight of OTUD6B is 34 kDa, 68450-1-Ig can detect the band around 34 kDa.(PMID:32323143)