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验证数据展示

  • WB analysis using 68450-1-Ig

    WB analysis using 68450-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68450-1-Ig (OTUD6B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of PC-3 using 68450-1-Ig

    IF Staining of PC-3 using 68450-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using OTUD6B antibody (68450-1-Ig, Clone: 1D1G1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

OTUD6B Monoclonal antibody

OTUD6B Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68450-1-Ig

Print datasheet

CloneNo. 1D1G1

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

DUBA5, DUBA 5, Deubiquitinase OTUD6B, CGI-77, CGI 77

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IF/ICC detected inPC-3 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68450-1-Ig targets OTUD6B in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen OTUD6B fusion protein Ag33607 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name OTU domain containing 6B
Synonyms DUBA5, DUBA 5, Deubiquitinase OTUD6B, CGI-77, CGI 77
Calculated Molecular Weight 293 aa, 34 kDa
Observed Molecular Weight 34-40 kDa
GenBank Accession NumberBC029760
Gene Symbol OTUD6B
Gene ID (NCBI) 51633
RRIDAB_3085162
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8N6M0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

OTUD6B, also known as DUBA5, belongs to a DUB subfamily characterized by an ovarian tumor domain (OTU). It's a functional deubiquitinating enzyme, a class of protease that specifically cleaves ubiquitin linkages. OTUD6B function may be connected to growth and proliferation. It has been reported that OTUD6B could significantly suppress HCC cells migration and metastasis. The calculated molecular weight of OTUD6B is 34 kDa, 68450-1-Ig can detect the band around 34 kDa.(PMID:32323143)

实验方案

Product Specific Protocols
WB protocol for OTUD6B antibody 68450-1-IgDownload protocol
IF protocol for OTUD6B antibody 68450-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols