验证数据展示
经过测试的应用
| Positive IF/ICC detected in | PC-3 cells |
For other applications, we recommend the unconjugated version of this antibody, 68450-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-68450 targets OTUD6B in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | OTUD6B fusion protein Ag33607 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | OTU domain containing 6B |
| 别名 | |
| 计算分子量 | 293 aa, 34 kDa |
| 观测分子量 | 34-40 kDa |
| GenBank蛋白编号 | BC029760 |
| 基因名称 | OTUD6B |
| Gene ID (NCBI) | 51633 |
| RRID | AB_3673018 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q8N6M0 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
OTUD6B, also known as DUBA5, belongs to a DUB subfamily characterized by an ovarian tumor domain (OTU). It's a functional deubiquitinating enzyme, a class of protease that specifically cleaves ubiquitin linkages. OTUD6B function may be connected to growth and proliferation. It has been reported that OTUD6B could significantly suppress HCC cells migration and metastasis. The calculated molecular weight of OTUD6B is 34 kDa, 68450-1-Ig can detect the band around 34 kDa.(PMID:32323143)
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 OTUD6B antibody CL488-68450 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |