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验证数据展示

  • WB analysis using 68450-1-Ig (same clone as 68450-1-PBS)

    WB analysis using 68450-1-Ig (same clone as 68450-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68450-1-Ig (OTUD6B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68450-1-PBS in a different storage buffer formulation.

  • IF Staining of PC-3 using 68450-1-Ig (same clone as 68450-1-PBS)

    IF Staining of PC-3 using 68450-1-Ig (same clone as 68450-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using OTUD6B antibody (68450-1-Ig, Clone: 1D1G1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68450-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50962-1

    Cytometric bead array standard curve of MP50962-1

    Cytometric bead array standard curve of MP50962-1, OTUD6B Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68450-1-PBS. Detection antibody: 68450-2-PBS. Standard:Ag33607. Range: 0.098-50 ng/mL.

  • Cytometric bead array standard curve of MP50962-2

    Cytometric bead array standard curve of MP50962-2

    Cytometric bead array standard curve of MP50962-2, OTUD6B Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68450-1-PBS. Detection antibody: 68450-3-PBS. Standard:Ag33607. Range: 0.098-25 ng/mL.

  • Cytometric bead array standard curve of MP50962-3

    Cytometric bead array standard curve of MP50962-3

    Cytometric bead array standard curve of MP50962-3, OTUD6B Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68450-1-PBS. Detection antibody: 68450-4-PBS. Standard:Ag33607. Range: 0.098-12.5 ng/mL.

OTUD6B Monoclonal antibody, PBS Only (Capture)

OTUD6B Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 68450-1-PBS

Print datasheet

CloneNo. 1D1G1

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

DUBA5, DUBA 5, Deubiquitinase OTUD6B, CGI-77, CGI 77

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

68450-1-PBS targets OTUD6B as part of a matched antibody pair:

MP50962-1: 68450-1-PBS capture and 68450-2-PBS detection (validated in Cytometric bead array)

MP50962-2: 68450-1-PBS capture and 68450-3-PBS detection (validated in Cytometric bead array)

MP50962-3: 68450-1-PBS capture and 68450-4-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen OTUD6B fusion protein Ag33607 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name OTU domain containing 6B
Synonyms DUBA5, DUBA 5, Deubiquitinase OTUD6B, CGI-77, CGI 77
Calculated Molecular Weight 293 aa, 34 kDa
Observed Molecular Weight 34-40 kDa
GenBank Accession NumberBC029760
Gene Symbol OTUD6B
Gene ID (NCBI) 51633
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8N6M0
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

OTUD6B, also known as DUBA5, belongs to a DUB subfamily characterized by an ovarian tumor domain (OTU). It's a functional deubiquitinating enzyme, a class of protease that specifically cleaves ubiquitin linkages. OTUD6B function may be connected to growth and proliferation. It has been reported that OTUD6B could significantly suppress HCC cells migration and metastasis. The calculated molecular weight of OTUD6B is 34 kDa, 68450-1-Ig can detect the band around 34 kDa.(PMID:32323143)