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验证数据展示

  • WB analysis using 68451-1-Ig (same clone as 68451-1-PBS)

    WB analysis using 68451-1-Ig (same clone as 68451-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68451-1-Ig (GIMAP7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68451-1-PBS in a different storage buffer formulation.

  • IP experiment of Jurkat using 68451-1-Ig (same clone as 68451-1-PBS)

    IP experiment of Jurkat using 68451-1-Ig (same clone as 68451-1-PBS)

    IP result of anti-GIMAP7 (IP:68451-1-Ig, 4ug; Detection:68451-1-Ig 1:2000) with Jurkat cells lysate 1560 ug. This data was developed using the same antibody clone with 68451-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 68451-1-Ig (same clone as 68451-1-PBS)

    IF Staining of HepG2 using 68451-1-Ig (same clone as 68451-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GIMAP7 antibody (68451-1-Ig, Clone: 3E4A6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68451-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50958-1

    Cytometric bead array standard curve of MP50958-1

    Cytometric bead array standard curve of MP50958-1, GIMAP7 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68451-1-PBS. Detection antibody: 68451-2-PBS. Standard:Ag10740. Range: 0.098-100 ng/mL.

  • Cytometric bead array standard curve of MP50958-2

    Cytometric bead array standard curve of MP50958-2

    Cytometric bead array standard curve of MP50958-2, GIMAP7 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68451-1-PBS. Detection antibody: 68451-3-PBS. Standard:Ag10740. Range: 0.098-100 ng/mL.

  • Cytometric bead array standard curve of MP50958-3

    Cytometric bead array standard curve of MP50958-3

    Cytometric bead array standard curve of MP50958-3, GIMAP7 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68451-1-PBS. Detection antibody: 68451-4-PBS. Standard:Ag10740. Range: 0.098-100 ng/mL.

  • Cytometric bead array standard curve of MP50958-4

    Cytometric bead array standard curve of MP50958-4

    Cytometric bead array standard curve of MP50958-4, GIMAP7 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68451-1-PBS. Detection antibody: 68451-5-PBS. Standard:Ag10740. Range: 0.098-100 ng/mL.

GIMAP7 Monoclonal antibody, PBS Only (Capture)

GIMAP7 Monoclonal Antibody for WB, IF/ICC, IP, Cytometric bead array, Indirect ELISA
Cat No. 68451-1-PBS

产品说明书

CloneNo. 3E4A6

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IF/ICC, IP, Cytometric bead array, Indirect ELISA

别名

Immunity-associated nucleotide 7 protein, IAN-7, IAN7, GTPase IMAP family member 7, 3E4A6

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

68451-1-PBS targets GIMAP7 as part of a matched antibody pair:

MP50958-1: 68451-1-PBS capture and 68451-2-PBS detection (validated in Cytometric bead array)

MP50958-2: 68451-1-PBS capture and 68451-3-PBS detection (validated in Cytometric bead array)

MP50958-3: 68451-1-PBS capture and 68451-4-PBS detection (validated in Cytometric bead array)

MP50958-4: 68451-1-PBS capture and 68451-5-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, IP, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 GIMAP7 fusion protein Ag10740 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 GTPase, IMAP family member 7
别名 Immunity-associated nucleotide 7 protein, IAN-7, IAN7, GTPase IMAP family member 7, 3E4A6
计算分子量 300 aa, 35 kDa
观测分子量 34 kDa
GenBank蛋白编号BC027613
基因名称 GIMAP7
Gene ID (NCBI) 168537
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ8NHV1
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GIMAP7, also known as hIAN7 or IAN 7, is a transmembrane protein located in the endoplasmic reticulum and Golgi apparatus. GIMAP7 can promote oxidative stress and apoptosis in ovarian granulosa cells in Polycystic ovary syndrome by inhibiting the SHH signalling pathway(PMID: 36581994). The MW of this protein is 34 kDa, and this antibody specially recognises the 34 kDa protein.