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验证数据展示

  • WB analysis using 68451-1-Ig

    WB analysis using 68451-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68451-1-Ig (GIMAP7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IP experiment of Jurkat using 68451-1-Ig

    IP experiment of Jurkat using 68451-1-Ig

    IP result of anti-GIMAP7 (IP:68451-1-Ig, 4ug; Detection:68451-1-Ig 1:2000) with Jurkat cells lysate 1560 ug.

  • IF Staining of HepG2 using 68451-1-Ig

    IF Staining of HepG2 using 68451-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GIMAP7 antibody (68451-1-Ig, Clone: 3E4A6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

GIMAP7 Monoclonal antibody

GIMAP7 Monoclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 68451-1-Ig

产品说明书

CloneNo. 3E4A6

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, IF/ICC, IP, ELISA

别名

GIMAP7, GTPase IMAP family member 7, GTPase, IMAP family member 7, hIAN7, IAN 7, IAN7

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inNK-92 cells, Jurkat cells, MOLT-4 cells, HUVEC cells
Positive IP detected inJurkat cells
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68451-1-Ig targets GIMAP7 in WB, IF/ICC, IP, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
经测试反应性 Human
免疫原 GIMAP7 fusion protein Ag10740 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 GTPase, IMAP family member 7
别名 GIMAP7, GTPase IMAP family member 7, GTPase, IMAP family member 7, hIAN7, IAN 7, IAN7
计算分子量 300 aa, 35 kDa
观测分子量 34 kDa
GenBank蛋白编号BC027613
基因名称 GIMAP7
Gene ID (NCBI) 168537
RRIDAB_3085163
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ8NHV1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GIMAP7, also known as hIAN7 or IAN 7, is a transmembrane protein located in the endoplasmic reticulum and Golgi apparatus. GIMAP7 can promote oxidative stress and apoptosis in ovarian granulosa cells in Polycystic ovary syndrome by inhibiting the SHH signalling pathway(PMID: 36581994). The MW of this protein is 34 kDa, and this antibody specially recognises the 34 kDa protein.

实验方案

Product Specific Protocols
WB protocol for GIMAP7 antibody 68451-1-IgDownload protocol
IF protocol for GIMAP7 antibody 68451-1-IgDownload protocol
IP protocol for GIMAP7 antibody 68451-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols