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GIMAP7 Monoclonal antibody

GIMAP7 Monoclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

3E4A6

Cat no : 68451-1-Ig

Print datasheet

Synonyms

GIMAP7, GTPase IMAP family member 7, GTPase, IMAP family member 7, hIAN7, IAN 7, IAN7

验证数据展示

  • WB analysis using 68451-1-Ig

    WB analysis using 68451-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68451-1-Ig (GIMAP7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IP experiment of Jurkat using 68451-1-Ig

    IP experiment of Jurkat using 68451-1-Ig

    IP result of anti-GIMAP7 (IP:68451-1-Ig, 4ug; Detection:68451-1-Ig 1:2000) with Jurkat cells lysate 1560 ug.

  • IF Staining of HepG2 using 68451-1-Ig

    IF Staining of HepG2 using 68451-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GIMAP7 antibody (68451-1-Ig, Clone: 3E4A6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inNK-92 cells, Jurkat cells, MOLT-4 cells, HUVEC cells
Positive IP detected inJurkat cells
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68451-1-Ig targets GIMAP7 in WB, IF/ICC, IP, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Tested Reactivity Human
Immunogen GIMAP7 fusion protein Ag10740 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name GTPase, IMAP family member 7
Synonyms GIMAP7, GTPase IMAP family member 7, GTPase, IMAP family member 7, hIAN7, IAN 7, IAN7
Calculated Molecular Weight 300 aa, 35 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC027613
Gene Symbol GIMAP7
Gene ID (NCBI) 168537
RRIDAB_3085163
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8NHV1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GIMAP7, also known as hIAN7 or IAN 7, is a transmembrane protein located in the endoplasmic reticulum and Golgi apparatus. GIMAP7 can promote oxidative stress and apoptosis in ovarian granulosa cells in Polycystic ovary syndrome by inhibiting the SHH signalling pathway(PMID: 36581994). The MW of this protein is 34 kDa, and this antibody specially recognises the 34 kDa protein.

实验方案

Product Specific Protocols
WB protocol for GIMAP7 antibody 68451-1-IgDownload protocol
IF protocol for GIMAP7 antibody 68451-1-IgDownload protocol
IP protocol for GIMAP7 antibody 68451-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols