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PPA2 Monoclonal antibody

PPA2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1C2E3

Cat no : 68468-1-Ig

Print datasheet

Synonyms

FLJ20459, HSPC124, PPA2, PPase 2, pyrophosphatase (inorganic) 2, Pyrophosphatase SID6 306, SID6 306

验证数据展示

  • WB analysis using 68468-1-Ig

    WB analysis using 68468-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68468-1-Ig (PPA2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • WB analysis of 4T1 using 68468-1-Ig

    WB analysis of 4T1 using 68468-1-Ig

    4T1 cells were subjected to SDS PAGE followed by western blot with 68468-1-Ig (PPA2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inU2OS cells, 4T1 cells, LNCaP cells, HeLa cells, HEK-293T cells, Jurkat cells, Raji cells, K-562 cells
Planning an IHC experiment? We recommend our IHCeasy PPA2 Ready-To-Use IHC Kit. PPA2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68468-1-Ig targets PPA2 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen PPA2 fusion protein Ag10125 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name pyrophosphatase (inorganic) 2
Synonyms FLJ20459, HSPC124, PPA2, PPase 2, pyrophosphatase (inorganic) 2, Pyrophosphatase SID6 306, SID6 306
Calculated Molecular Weight305aa,35 kDa; 334aa,38 kDa
Observed Molecular Weight34 kDa
GenBank Accession NumberBC057219
Gene Symbol PPA2
Gene ID (NCBI) 27068
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9H2U2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Pyrophosphatase 2 (PPA2) is a mitochondrial-located pyrophosphatase (PPase) that hydrolyzes inorganic pyrophosphate (PPi), generated by numerous nucleotide-dependent reactions, into two orthophosphate molecules (PMID: 34400813). PPA2 is a cardiomyopathy-associated protein, which has a greater physiological importance in mitochondrial function (PMID: 27523597).

实验方案

Product Specific Protocols
WB protocol for PPA2 antibody 68468-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols