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验证数据展示

  • WB analysis of HEK-293 using 68468-1-Ig (same clone as 68468-1-PBS)

    WB analysis of HEK-293 using 68468-1-Ig (same clone as 68468-1-PBS)

    WB result of PPA2 antibody (68468-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPA2 transfected HEK-293 cells. This data was developed using the same antibody clone with 68468-1-PBS in a different storage buffer formulation.

  • WB analysis using 68468-1-Ig (same clone as 68468-1-PBS)

    WB analysis using 68468-1-Ig (same clone as 68468-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68468-1-Ig (PPA2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68468-1-PBS in a different storage buffer formulation.

  • WB analysis of 4T1 using 68468-1-Ig (same clone as 68468-1-PBS)

    WB analysis of 4T1 using 68468-1-Ig (same clone as 68468-1-PBS)

    4T1 cells were subjected to SDS PAGE followed by western blot with 68468-1-Ig (PPA2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68468-1-PBS in a different storage buffer formulation.

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  • KD/KO验证

PPA2 Monoclonal antibody, PBS Only

PPA2 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 68468-1-PBS

产品说明书

CloneNo. 1C2E3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, Indirect ELISA

别名

1C2E3, EC:3.6.1.1, HSPC124, PPase 2, Pyrophosphatase SID6 306

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PPA2 Ready-To-Use IHC Kit. PPA2 primary antibody included.

产品信息

68468-1-PBS targets PPA2 in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag10125

Product name: Recombinant human PPA2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-305 aa of BC057219

Sequence: MSALLRLLRTGAPAAACLRLGTSAGTGSRRAMALYHTEERGQPCSQNYRLFFKNVTGHYISPFHDIPLKVNSKEENGIPMKKARNDEYENLFNMIVEIPRWTNAKMEIATKEPMNPIKQYVKDGKLRYVANIFPYKGYIWNYGTLPQILSCGEVIHVKILGILALIDEGETDWKLIAINANDPEASKFHDIDDVKKFKPGYLEATLNWFRLYKVPDGKPENQFAFNGEFKNKAFALEVIKSTHQCWKALLMKKCNGGAINCTNVQISDSPFRCTQEEARSLVESVSSSPNKESNEEEQVWHFLGK

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 pyrophosphatase (inorganic) 2
别名 1C2E3, EC:3.6.1.1, HSPC124, PPase 2, Pyrophosphatase SID6 306
计算分子量305aa,35 kDa; 334aa,38 kDa
观测分子量34 kDa
GenBank蛋白编号BC057219
基因名称 PPA2
Gene ID (NCBI) 27068
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9H2U2
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Pyrophosphatase 2 (PPA2) is a mitochondrial-located pyrophosphatase (PPase) that hydrolyzes inorganic pyrophosphate (PPi), generated by numerous nucleotide-dependent reactions, into two orthophosphate molecules (PMID: 34400813). PPA2 is a cardiomyopathy-associated protein, which has a greater physiological importance in mitochondrial function (PMID: 27523597).

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