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Lamin B2 Monoclonal antibody

Lamin B2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2C7B1

Cat No : 68475-1-Ig

Print datasheet

Synonyms

LAMB2, lamin B2, LMN2, LMNB2

验证数据展示

  • WB analysis using 68475-1-Ig

    WB analysis using 68475-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68475-1-Ig (Lamin B2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inA549 cells, LNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68475-1-Ig targets Lamin B2 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen Lamin B2 fusion protein Ag33296 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name lamin B2
Synonyms LAMB2, lamin B2, LMN2, LMNB2
Calculated Molecular Weight 68 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC006551
Gene Symbol LMNB2
Gene ID (NCBI) 84823
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ03252
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Lamins are nuclear membrane structural components that are important in structural integrity of the nucleus and may also interact with chromatin (PMID: 33033404). Research studies show that lamin B2 knockout mice exhibit neuronal developmental defects and that both proteins are essential for typical brain development (PMID: 20145110). Mutations in Lamin B2 can result in a susceptibility to developing acquired partial lipodystrophy, a rare disorder characterized by the progressive loss of subcutaneous fat in a bilaterally symmetrical fashion (PMID: 16826530).

实验方案

Product Specific Protocols
WB protocol for Lamin B2 antibody 68475-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols