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验证数据展示

  • WB analysis using 68480-1-Ig

    WB analysis using 68480-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68480-1-Ig (DIABLO antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis using 68480-1-Ig

    WB analysis using 68480-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68480-1-Ig (DIABLO antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 68480-1-Ig

    IF Staining of HepG2 using 68480-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Smac/DIABLO antibody (68480-1-Ig, Clone: 2F12C9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).

Smac/DIABLO Monoclonal antibody

Smac/DIABLO Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68480-1-Ig

产品说明书

CloneNo. 2F12C9

宿主/亚型

Mouse / IgG1

种属反应性

human, rat

应用

WB, IF/ICC, ELISA

别名

DIABLO, Diablo IAP-binding mitochondrial protein, cleaved form, Diablo IAP-binding mitochondrial protein, Diablo homolog, mitochondrial, DFNA64

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inU2OS cells, K-562 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, HSC-T6 cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68480-1-Ig targets Smac/DIABLO in WB, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, rat
免疫原 Smac/DIABLO fusion protein Ag32774 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 diablo homolog (Drosophila)
别名 DIABLO, Diablo IAP-binding mitochondrial protein, cleaved form, Diablo IAP-binding mitochondrial protein, Diablo homolog, mitochondrial, DFNA64
计算分子量 27 kDa
观测分子量 20 kDa
GenBank蛋白编号BC011909
基因名称 DIABLO
Gene ID (NCBI) 56616
RRIDAB_3085191
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NR28
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Smac/DIABLO is a mitochondrial protein that promotes apoptosis by neutralizing members of the IAP family. SMAC/DIABLO is ubiquitously expressed in normal tissues, and the expression of SMAC messenger RNA (mRNA) in adult testis is the highest .Smac/DIABLO encodes a 239 amino acid protein (~27 kDa). The first 55 amino acids are a mitochondrial targeting signal peptide that is cleaved to generate mature Smac-DIABLO (~21 kDa). When released from mitochondria, Smac-DIABLO acts as a natural antagonist of inhibitor of apoptosis proteins (IAPs). Smac-DIABLO antagonistic activity is based on its N-terminal tetrapeptide (AVPI) that binds baculoviral IAP repeat (BIR) domains of IAPs, releasing their inhibitory effects on both initiator and effector caspases, thus promoting cell death. (PMID: 32325691, PMID: 32575872,PMID: 25650938)

实验方案

Product Specific Protocols
WB protocol for Smac/DIABLO antibody 68480-1-IgDownload protocol
IF protocol for Smac/DIABLO antibody 68480-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols