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DIABLO Monoclonal antibody, PBS Only

DIABLO Monoclonal Antibody for WB,Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat

Applications

WB,Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F12C9

Cat No : 68480-1-PBS

Print datasheet

Synonyms

DIABLO, diablo homolog (Drosophila), Diablo homolog, mitochondrial, DIABLO S, FLJ10537, FLJ25049, SMAC, SMAC3

验证数据展示

  • WB analysis using 68480-1-Ig (same clone as 68480-1-PBS)

    WB analysis using 68480-1-Ig (same clone as 68480-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68480-1-Ig (DIABLO antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68480-1-PBS in a different storage buffer formulation.

  • WB analysis using 68480-1-Ig (same clone as 68480-1-PBS)

    WB analysis using 68480-1-Ig (same clone as 68480-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68480-1-Ig (DIABLO antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68480-1-PBS in a different storage buffer formulation.

产品信息

68480-1-PBS targets DIABLO in WB, Indirect ELISA applications and shows reactivity with Human, rat samples.

Tested Applications WB,Indirect ELISA Application Description
Tested Reactivity Human, rat
Immunogen DIABLO fusion protein Ag32774 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name diablo homolog (Drosophila)
Synonyms DIABLO, diablo homolog (Drosophila), Diablo homolog, mitochondrial, DIABLO S, FLJ10537, FLJ25049, SMAC, SMAC3
Calculated Molecular Weight 27 kDa
Observed Molecular Weight 20 kDa
GenBank Accession NumberBC011909
Gene Symbol DIABLO
Gene ID (NCBI) 56616
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NR28
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Smac/DIABLO is a mitochondrial protein that promotes apoptosis by neutralizing members of the IAP family. SMAC/DIABLO is ubiquitously expressed in normal tissues, and the expression of SMAC messenger RNA (mRNA) in adult testis is the highest .Smac/DIABLO encodes a 239 amino acid protein (~27 kDa). The first 55 amino acids are a mitochondrial targeting signal peptide that is cleaved to generate mature Smac-DIABLO (~21 kDa). When released from mitochondria, Smac-DIABLO acts as a natural antagonist of inhibitor of apoptosis proteins (IAPs). Smac-DIABLO antagonistic activity is based on its N-terminal tetrapeptide (AVPI) that binds baculoviral IAP repeat (BIR) domains of IAPs, releasing their inhibitory effects on both initiator and effector caspases, thus promoting cell death. (PMID: 32325691, PMID: 32575872,PMID: 25650938)