FLAD1 Monoclonal antibody
FLAD1 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
1F9A8
验证数据展示
经过测试的应用
| Positive WB detected in | LNCaP cells, U2OS cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68491-1-Ig targets FLAD1 in WB, ELISA applications and shows reactivity with Human samples.
| Tested Applications | WB, ELISA Application Description |
| Tested Reactivity | Human |
| Immunogen | FLAD1 fusion protein Ag6845 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | FAD1 flavin adenine dinucleotide synthetase homolog (S. cerevisiae) |
| Synonyms | FAD pyrophosphorylase, FAD synthase, FAD1, FADS, FLAD1, FMN adenylyltransferase, PP591, RP11 307C12.7 |
| Calculated Molecular Weight | 446 aa, 49 kDa |
| Observed Molecular Weight | 50 kDa |
| GenBank Accession Number | BC011378 |
| Gene Symbol | FLAD1 |
| Gene ID (NCBI) | 80308 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q8NFF5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FLAD1 is a protein-coding gene for flavin adenine dinucleotide synthetase (FADS), a key enzyme in the FAD biosynthesis process which contains an N-terminal molybdopterin -binding (MPTb) domain and a C-terminal domain (FADS domain) (PMID: 32714079). Alternative splicing of the human FLAD1 gene generates different isoforms of the enzyme FAD synthase. ~60 and ~50 kDa bands correspond to the expected mitochondrial FADS1 and cytosolic FADS2 proteins, respectively (PMID: 29316637).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FLAD1 antibody 68491-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
