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验证数据展示

  • WB analysis using 68491-1-Ig (same clone as 68491-1-PBS)

    WB analysis using 68491-1-Ig (same clone as 68491-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68491-1-Ig (FLAD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68491-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 68491-1-Ig (same clone as 68491-1-PBS)

    WB analysis of HEK-293 using 68491-1-Ig (same clone as 68491-1-PBS)

    WB result of FLAD1 antibody (68491-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FLAD1 transfected HEK-293 cells. This data was developed using the same antibody clone with 68491-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

FLAD1 Monoclonal antibody, PBS Only

FLAD1 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 68491-1-PBS

产品说明书

CloneNo. 1F9A8

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, Indirect ELISA

别名

FAD1, FAD synthase region, FAD synthase, FAD pyrophosphorylase, EC:2.7.7.2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy FLAD1 Ready-To-Use IHC Kit. FLAD1 primary antibody included.

产品信息

68491-1-PBS targets FLAD1 in WB, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human
免疫原 FLAD1 fusion protein Ag6845 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 FAD1 flavin adenine dinucleotide synthetase homolog (S. cerevisiae)
别名 FAD1, FAD synthase region, FAD synthase, FAD pyrophosphorylase, EC:2.7.7.2
计算分子量 446 aa, 49 kDa
观测分子量50 kDa
GenBank蛋白编号BC011378
基因名称 FLAD1
Gene ID (NCBI) 80308
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ8NFF5
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FLAD1 is a protein-coding gene for flavin adenine dinucleotide synthetase (FADS), a key enzyme in the FAD biosynthesis process which contains an N-terminal molybdopterin -binding (MPTb) domain and a C-terminal domain (FADS domain) (PMID: 32714079). Alternative splicing of the human FLAD1 gene generates different isoforms of the enzyme FAD synthase. ~60 and ~50 kDa bands correspond to the expected mitochondrial FADS1 and cytosolic FADS2 proteins, respectively (PMID: 29316637).