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验证数据展示

  • WB analysis using 68500-1-Ig

    WB analysis using 68500-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68500-1-Ig (PSPH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of A375 using 68500-1-Ig

    WB analysis of A375 using 68500-1-Ig

    WB result of PSPH antibody (68500-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSPH transfected A375 cells.

  • IP experiment of HL-60 using 68500-1-Ig

    IP experiment of HL-60 using 68500-1-Ig

    IP result of anti-PSPH (IP:68500-1-Ig, 4ug; Detection:68500-1-Ig 1:5000) with HL-60 cells lysate 2440 ug.

  • IHC staining of mouse spleen using 68500-1-Ig

    IHC staining of mouse spleen using 68500-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 68500-1-Ig (PSPH antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 68500-1-Ig

    IF Staining of HepG2 using 68500-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PSPH antibody (68500-1-Ig, Clone: 1B12G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Featured Product
  • KD/KO验证

PSPH Monoclonal antibody

PSPH Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 68500-1-Ig

产品说明书

CloneNo. 1B12G3

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

L 3 phosphoserine phosphatase, phosphoserine phosphatase, PSP, PSPase, PSPH

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, A375 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IP detected inHL-60 cells
Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68500-1-Ig targets PSPH in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 PSPH fusion protein Ag6070 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 phosphoserine phosphatase
别名 L 3 phosphoserine phosphatase, phosphoserine phosphatase, PSP, PSPase, PSPH
计算分子量 25 kDa
观测分子量25 kDa
GenBank蛋白编号BC063614
基因名称 PSPH
Gene ID (NCBI) 5723
RRIDAB_3085211
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP78330
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSPH (phosphoserine phosphatase) is an enzyme, which is involved in the process of L-serine biosynthesis. PSPH mainly plays role in multiple aspects of cell behaviours such as proliferation and differentiation by producing precursors for the biosynthesis of diverse compounds including neurotransmitters, glycolipids and thymidine (PMID: 11237721, PMID: 19963421). Additionally, augmented PSPH level is correlated with the prognosis in multiple cancers including cutaneous squamous cell carcinoma (PMID: 21726982), breast cancer (PMID: 28931725), non-small cell lung cancer (PMID: 30662358), colorectal cancer (PMID: 24146633) and hepatocellular carcinoma (PMID: 25793315).

实验方案

Product Specific Protocols
WB protocol for PSPH antibody 68500-1-IgDownload protocol
IHC protocol for PSPH antibody 68500-1-IgDownload protocol
IF protocol for PSPH antibody 68500-1-IgDownload protocol
IP protocol for PSPH antibody 68500-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols