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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 68500-1-Ig (same clone as 68500-1-PBS)

    WB analysis using 68500-1-Ig (same clone as 68500-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68500-1-Ig (PSPH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68500-1-PBS in a different storage buffer formulation.

  • WB analysis of A375 using 68500-1-Ig (same clone as 68500-1-PBS)

    WB analysis of A375 using 68500-1-Ig (same clone as 68500-1-PBS)

    WB result of PSPH antibody (68500-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSPH transfected A375 cells. This data was developed using the same antibody clone with 68500-1-PBS in a different storage buffer formulation.

  • IP experiment of HL-60 using 68500-1-Ig (same clone as 68500-1-PBS)

    IP experiment of HL-60 using 68500-1-Ig (same clone as 68500-1-PBS)

    IP result of anti-PSPH (IP:68500-1-Ig, 4ug; Detection:68500-1-Ig 1:5000) with HL-60 cells lysate 2440 ug. This data was developed using the same antibody clone with 68500-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 68500-1-Ig (same clone as 68500-1-PBS)

    IHC staining of mouse spleen using 68500-1-Ig (same clone as 68500-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 68500-1-Ig (PSPH antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68500-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 68500-1-Ig (same clone as 68500-1-PBS)

    IF Staining of HepG2 using 68500-1-Ig (same clone as 68500-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PSPH antibody (68500-1-Ig, Clone: 1B12G3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68500-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

PSPH Monoclonal antibody, PBS Only

PSPH Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 68500-1-PBS

产品说明书

CloneNo. 1B12G3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

PSPase, PSP, O-phosphoserine phosphohydrolase, L-3-phosphoserine phosphatase, EC:3.1.3.3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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68500-1-Ig

产品信息

68500-1-PBS targets PSPH in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PSPH fusion protein Ag6070 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 phosphoserine phosphatase
别名 PSPase, PSP, O-phosphoserine phosphohydrolase, L-3-phosphoserine phosphatase, EC:3.1.3.3
计算分子量 25 kDa
观测分子量25 kDa
GenBank蛋白编号BC063614
基因名称 PSPH
Gene ID (NCBI) 5723
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP78330
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PSPH (phosphoserine phosphatase) is an enzyme, which is involved in the process of L-serine biosynthesis. PSPH mainly plays role in multiple aspects of cell behaviours such as proliferation and differentiation by producing precursors for the biosynthesis of diverse compounds including neurotransmitters, glycolipids and thymidine (PMID: 11237721, PMID: 19963421). Additionally, augmented PSPH level is correlated with the prognosis in multiple cancers including cutaneous squamous cell carcinoma (PMID: 21726982), breast cancer (PMID: 28931725), non-small cell lung cancer (PMID: 30662358), colorectal cancer (PMID: 24146633) and hepatocellular carcinoma (PMID: 25793315).