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验证数据展示

  • WB analysis using 68506-1-Ig

    WB analysis using 68506-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68506-1-Ig (ATP6V0D1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 68506-1-Ig

    IP experiment of HeLa using 68506-1-Ig

    IP result of anti-ATP6V0D1 (IP:68506-1-Ig, 4ug; Detection:68506-1-Ig 1:3000) with HeLa cells lysate 1520 ug.

  • IF Staining of HeLa using 68506-1-Ig

    IF Staining of HeLa using 68506-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ATP6V0D1 antibody (68506-1-Ig, Clone: 1G7A12 ) at dilution of 1:2400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IHC staining of mouse kidney using 68506-1-Ig

    IHC staining of mouse kidney using 68506-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68506-1-Ig (ATP6V0D1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 68506-1-Ig

    IHC staining of mouse kidney using 68506-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68506-1-Ig (ATP6V0D1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ATP6V0D1 Monoclonal antibody

ATP6V0D1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 68506-1-Ig

产品说明书

CloneNo. 1G7A12

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Rat, Pig, Rabbit, Chicken, Zebrafish

应用

WB, IHC, IF/ICC, IP, ELISA

别名

32 kDa accessory protein, ATP6D, ATP6DV, ATP6V0D1, P39, V ATPase AC39 subunit, V ATPase subunit d 1, VATX, VMA6, VPATPD

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, K-562 cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue, chicken brain tissue, zebrafish tissue
Positive IP detected inHeLa cells
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:20000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:1200-1:4800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68506-1-Ig targets ATP6V0D1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit, Chicken, Zebrafish samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 Human, Mouse, Rat, Pig, Rabbit, Chicken, Zebrafish
免疫原 ATP6V0D1 fusion protein Ag13012 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ATPase, H+ transporting, lysosomal 38kDa, V0 subunit d1
别名 32 kDa accessory protein, ATP6D, ATP6DV, ATP6V0D1, P39, V ATPase AC39 subunit, V ATPase subunit d 1, VATX, VMA6, VPATPD
计算分子量 351 aa, 40 kDa
观测分子量37-41 kDa
GenBank蛋白编号BC008861
基因名称 ATP6V0D1
Gene ID (NCBI) 9114
RRIDAB_3085216
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP61421
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATP6V0D1(V-type proton ATPase subunit d 1) is also named as ATP6D, VPATPD and belongs to the V-ATPase V0D/AC39 subunit family.It is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system.

实验方案

Product Specific Protocols
WB protocol for ATP6V0D1 antibody 68506-1-IgDownload protocol
IHC protocol for ATP6V0D1 antibody 68506-1-IgDownload protocol
IF protocol for ATP6V0D1 antibody 68506-1-IgDownload protocol
IP protocol for ATP6V0D1 antibody 68506-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols