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ATP6V0D1 Monoclonal antibody, PBS Only

ATP6V0D1 Monoclonal Antibody for WB,IP,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig, Rabbit, Chicken, Zebrafish

Applications

WB,IP,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

1G7A12

Cat No : 68506-1-PBS

Print datasheet

Synonyms

32 kDa accessory protein, ATP6D, ATP6DV, ATP6V0D1, P39, V ATPase AC39 subunit, V ATPase subunit d 1, VATX, VMA6, VPATPD

验证数据展示

  • WB analysis using 68506-1-Ig (same clone as 68506-1-PBS)

    WB analysis using 68506-1-Ig (same clone as 68506-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68506-1-Ig (ATP6V0D1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68506-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 68506-1-Ig (same clone as 68506-1-PBS)

    IP experiment of HeLa using 68506-1-Ig (same clone as 68506-1-PBS)

    IP result of anti-ATP6V0D1 (IP:68506-1-Ig, 4ug; Detection:68506-1-Ig 1:3000) with HeLa cells lysate 1520 ug. This data was developed using the same antibody clone with 68506-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 68506-1-Ig (same clone as 68506-1-PBS)

    IF Staining of HeLa using 68506-1-Ig (same clone as 68506-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ATP6V0D1 antibody (68506-1-Ig, Clone: 1G7A12 ) at dilution of 1:2400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68506-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 68506-1-Ig (same clone as 68506-1-PBS)

    IHC staining of mouse kidney using 68506-1-Ig (same clone as 68506-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68506-1-Ig (ATP6V0D1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68506-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 68506-1-Ig (same clone as 68506-1-PBS)

    IHC staining of mouse kidney using 68506-1-Ig (same clone as 68506-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68506-1-Ig (ATP6V0D1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68506-1-PBS in a different storage buffer formulation.

产品信息

68506-1-PBS targets ATP6V0D1 in WB, IP, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit, Chicken, Zebrafish samples.

Tested Applications WB,IP,Indirect ELISA,IHC,IF Application Description
Tested Reactivity Human, Mouse, Rat, Pig, Rabbit, Chicken, Zebrafish
Immunogen ATP6V0D1 fusion protein Ag13012 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ATPase, H+ transporting, lysosomal 38kDa, V0 subunit d1
Synonyms 32 kDa accessory protein, ATP6D, ATP6DV, ATP6V0D1, P39, V ATPase AC39 subunit, V ATPase subunit d 1, VATX, VMA6, VPATPD
Calculated Molecular Weight 351 aa, 40 kDa
Observed Molecular Weight37-41 kDa
GenBank Accession NumberBC008861
Gene Symbol ATP6V0D1
Gene ID (NCBI) 9114
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP61421
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

ATP6V0D1(V-type proton ATPase subunit d 1) is also named as ATP6D, VPATPD and belongs to the V-ATPase V0D/AC39 subunit family.It is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system.