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ATP6V0D1 Monoclonal antibody, PBS Only

ATP6V0D1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig, Rabbit, Chicken, Zebrafish

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G7A12

Cat no : 68506-1-PBS

Print datasheet

Synonyms

32 kDa accessory protein, ATP6D, ATP6DV, ATP6V0D1, P39, V ATPase AC39 subunit, V ATPase subunit d 1, VATX, VMA6, VPATPD

验证数据展示

  • WB analysis using 68506-1-Ig (same clone as 68506-1-PBS)

    WB analysis using 68506-1-Ig (same clone as 68506-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68506-1-Ig (ATP6V0D1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68506-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 68506-1-Ig (same clone as 68506-1-PBS)

    IP experiment of HeLa using 68506-1-Ig (same clone as 68506-1-PBS)

    IP result of anti-ATP6V0D1 (IP:68506-1-Ig, 4ug; Detection:68506-1-Ig 1:3000) with HeLa cells lysate 1520 ug. This data was developed using the same antibody clone with 68506-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 68506-1-Ig (same clone as 68506-1-PBS)

    IF Staining of HeLa using 68506-1-Ig (same clone as 68506-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ATP6V0D1 antibody (68506-1-Ig, Clone: 1G7A12 ) at dilution of 1:2400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68506-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 68506-1-Ig (same clone as 68506-1-PBS)

    IHC staining of mouse kidney using 68506-1-Ig (same clone as 68506-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68506-1-Ig (ATP6V0D1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68506-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 68506-1-Ig (same clone as 68506-1-PBS)

    IHC staining of mouse kidney using 68506-1-Ig (same clone as 68506-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68506-1-Ig (ATP6V0D1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68506-1-PBS in a different storage buffer formulation.

产品信息

68506-1-PBS targets ATP6V0D1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit, Chicken, Zebrafish samples.

Tested Applications WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity Human, Mouse, Rat, Pig, Rabbit, Chicken, Zebrafish
Immunogen ATP6V0D1 fusion protein Ag13012 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ATPase, H+ transporting, lysosomal 38kDa, V0 subunit d1
Synonyms 32 kDa accessory protein, ATP6D, ATP6DV, ATP6V0D1, P39, V ATPase AC39 subunit, V ATPase subunit d 1, VATX, VMA6, VPATPD
Calculated Molecular Weight 351 aa, 40 kDa
Observed Molecular Weight37-41 kDa
GenBank Accession NumberBC008861
Gene Symbol ATP6V0D1
Gene ID (NCBI) 9114
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP61421
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ATP6V0D1(V-type proton ATPase subunit d 1) is also named as ATP6D, VPATPD and belongs to the V-ATPase V0D/AC39 subunit family.It is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system.