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验证数据展示

  • WB analysis using 68516-1-Ig (same clone as 68516-1-PBS)

    WB analysis using 68516-1-Ig (same clone as 68516-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68516-1-Ig (ATL3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68516-1-PBS in a different storage buffer formulation.

  • WB analysis of Jurkat using 68516-1-Ig (same clone as 68516-1-PBS)

    WB analysis of Jurkat using 68516-1-Ig (same clone as 68516-1-PBS)

    WB result of ATL3 antibody (68516-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATL3 transfected Jurkat cells. This data was developed using the same antibody clone with 68516-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 68516-1-Ig (same clone as 68516-1-PBS)

    IF Staining of HeLa using 68516-1-Ig (same clone as 68516-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATL3 antibody (68516-1-Ig, Clone: 2E7C4 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 68516-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50703-1

    Cytometric bead array standard curve of MP50703-1

    Cytometric bead array standard curve of MP50703-1, ATL3 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68516-2-PBS. Detection antibody: 68516-1-PBS. Standard:Ag10248. Range: 0.781-100 ng/mL.

  • Featured Product
  • KD/KO验证

ATL3 Monoclonal antibody, PBS Only (Detector)

ATL3 Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 68516-1-PBS

产品说明书

CloneNo. 2E7C4

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, rabbit

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

EC:3.6.5.-, Atlastin-3, Atlastin 3, 2E7C4

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

68516-1-PBS targets ATL3 as part of a matched antibody pair:

MP50703-1: 68516-2-PBS capture and 68516-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, rabbit
免疫原 ATL3 fusion protein Ag10248 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 atlastin GTPase 3
别名 EC:3.6.5.-, Atlastin-3, Atlastin 3, 2E7C4
计算分子量 541 aa, 61 kDa
观测分子量 61 kDa
GenBank蛋白编号BC077727
基因名称 ATL3
Gene ID (NCBI) 25923
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ6DD88
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ATL3, belonging to a class of dynamin-like GTPase, was reported to mediate ER fusion. ATL3 is required for the proper formation of the network of interconnected tubules of the endoplasmic reticulum. ATL3 functions as a receptor for ER-phagy, promoting tubular ER degradation upon starvation. Mutations in this gene may be associated with hereditary sensory neuropathy type IF.