验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells, K-562 cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68521-1-Ig targets RNF8 in WB, ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human, mouse, rat, rabbit, pig |
| 免疫原 | RNF8 fusion protein Ag5292 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | ring finger protein 8 |
| 别名 | FLJ12013, KIAA0646, ring finger protein 8, RNF8 |
| 计算分子量 | 485 aa, 56 kDa |
| 观测分子量 | 65-70 kDa |
| GenBank蛋白编号 | BC007517 |
| 基因名称 | RNF8 |
| Gene ID (NCBI) | 9025 |
| RRID | AB_3085230 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | O76064 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RNF8, also named as KIAA0646 or E3 ubiquitin-protein ligase RNF8, is a 485 amino acid protein, which contains one RING-type zinc finger and one FHA domain. RNF8 localizes in the nucleus and belongs to the RNF8 family. E2-E3 ubiquitin ligase complex is composed of the RNF8 homodimer and a E2 heterodimer of UBE2N and UBE2V2. E3 ubiquitin-protein ligase that plays a key role in DNA damage signaling via 2 distinct roles: by mediating the 'Lys-63'-linked ubiquitination of histones H2A and H2AX and promoting the recruitment of DNA repair proteins at double-strand breaks (DSBs) sites, and by catalyzing 'Lys-48'-linked ubiquitination to remove target proteins from DNA damage sites.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RNF8 antibody 68521-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |