SLC1A5/ASCT2 Monoclonal antibody
SLC1A5/ASCT2 Monoclonal Antibody for WB, FC, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human
Applications
WB, FC, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
1C4E1
验证数据展示
经过测试的应用
Positive WB detected in | A431 cells, PNGase F-treated A431 cells, A549 cells, PNGase F-treated A549 cells, HT-29 cells, PNGase F-treated HT-29 cells |
Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
68540-1-Ig targets SLC1A5/ASCT2 in WB, FC, IHC, ELISA applications and shows reactivity with Human samples.
Tested Applications | WB, FC, IHC, ELISA Application Description |
Tested Reactivity | Human |
Immunogen | SLC1A5/ASCT2 fusion protein Ag16837 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | solute carrier family 1 (neutral amino acid transporter), member 5 |
Synonyms | AAAT, ASCT2, ATB(0), ATBO, Baboon M7 virus receptor, FLJ31068, M7V1, M7VS1, R16, RDR, RDRC, SLC1A5 |
Calculated Molecular Weight | 541 aa, 57 kDa |
Observed Molecular Weight | 55-70 kDa |
GenBank Accession Number | BC000062 |
Gene Symbol | SLC1A5 |
Gene ID (NCBI) | 6510 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | Q15758 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for SLC1A5/ASCT2 antibody 68540-1-Ig | Download protocol |
IHC protocol for SLC1A5/ASCT2 antibody 68540-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |