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SLC1A5/ASCT2 Monoclonal antibody, PBS Only

SLC1A5/ASCT2 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C4E1

Cat no : 68540-1-PBS

Print datasheet

Synonyms

SLC1A5, Baboon M7 virus receptor, ATBO, ATB(0), ASCT2

验证数据展示

  • WB analysis using 68540-1-Ig (same clone as 68540-1-PBS)

    WB analysis using 68540-1-Ig (same clone as 68540-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68540-1-Ig (SLC1A5/ASCT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68540-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 68540-1-Ig (same clone as 68540-1-PBS)

    IHC staining of human stomach cancer using 68540-1-Ig (same clone as 68540-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68540-1-Ig (RDRC antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68540-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 68540-1-Ig (same clone as 68540-1-PBS)

    IF Staining of HEK-293 using 68540-1-Ig (same clone as 68540-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using SLC1A5/ASCT2 antibody (68540-1-Ig, Clone: 1C4E1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68540-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 68540-1-Ig (same clone as 68540-1-PBS)

    FC experiment of HeLa using 68540-1-Ig (same clone as 68540-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human SLC1A5/ASCT2 (68540-1-Ig, Clone:1C4E1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68540-1-PBS in a different storage buffer formulation.

产品信息

68540-1-PBS targets SLC1A5/ASCT2 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human
Immunogen SLC1A5/ASCT2 fusion protein Ag16837 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name solute carrier family 1 (neutral amino acid transporter), member 5
Synonyms SLC1A5, Baboon M7 virus receptor, ATBO, ATB(0), ASCT2
Calculated Molecular Weight 541 aa, 57 kDa
Observed Molecular Weight 55-70 kDa
GenBank Accession NumberBC000062
Gene Symbol SLC1A5/ASCT2
Gene ID (NCBI) 6510
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ15758
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SLC1A5 (Solute Carrier Family 1, member 5), also named as ASCT2, a major glutamine transporter belonging to the SLC1 family and localized in the plasma membrane of several body districts. Consistent with the functions exerted by glutamine, SLC1A5 is involved in uptake of essential amino acids, activation of mTORC1 and glutamine-dependent tumor cell survival and growth. SLC1A5 is highly expressed in various malignancies and plays a critical role in the transformation, growth and survival of cancer cells (PMID: 30234109). High SLC1A5 expression is associated with poor prognosis in clear-cell renal cell carcinoma (PMID: 26599282).