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Host
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Species Reactivity
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis using 68540-1-Ig (same clone as 68540-1-PBS)

    WB analysis using 68540-1-Ig (same clone as 68540-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68540-1-Ig (SLC1A5/ASCT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68540-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 68540-1-Ig (same clone as 68540-1-PBS)

    IHC staining of human stomach cancer using 68540-1-Ig (same clone as 68540-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68540-1-Ig (RDRC antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68540-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SLC1A5/ASCT2 antibody included
  • IF Staining of HEK-293 using 68540-1-Ig (same clone as 68540-1-PBS)

    IF Staining of HEK-293 using 68540-1-Ig (same clone as 68540-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using SLC1A5/ASCT2 antibody (68540-1-Ig, Clone: 1C4E1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68540-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 68540-1-Ig (same clone as 68540-1-PBS)

    FC experiment of HeLa using 68540-1-Ig (same clone as 68540-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human SLC1A5/ASCT2 (68540-1-Ig, Clone:1C4E1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68540-1-PBS in a different storage buffer formulation.

SLC1A5/ASCT2 Monoclonal antibody, PBS Only

SLC1A5/ASCT2 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 68540-1-PBS

产品说明书

CloneNo. 1C4E1

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

SLC1A5, Baboon M7 virus receptor, ATBO, ATB(0), ASCT2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy SLC1A5/ASCT2 Ready-To-Use IHC Kit. SLC1A5/ASCT2 primary antibody included.

产品信息

68540-1-PBS targets SLC1A5/ASCT2 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 SLC1A5/ASCT2 fusion protein Ag16837 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 solute carrier family 1 (neutral amino acid transporter), member 5
别名 SLC1A5, Baboon M7 virus receptor, ATBO, ATB(0), ASCT2
计算分子量 541 aa, 57 kDa
观测分子量 55-70 kDa
GenBank蛋白编号BC000062
基因名称 SLC1A5/ASCT2
Gene ID (NCBI) 6510
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ15758
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SLC1A5 (Solute Carrier Family 1, member 5), also named as ASCT2, a major glutamine transporter belonging to the SLC1 family and localized in the plasma membrane of several body districts. Consistent with the functions exerted by glutamine, SLC1A5 is involved in uptake of essential amino acids, activation of mTORC1 and glutamine-dependent tumor cell survival and growth. SLC1A5 is highly expressed in various malignancies and plays a critical role in the transformation, growth and survival of cancer cells (PMID: 30234109). High SLC1A5 expression is associated with poor prognosis in clear-cell renal cell carcinoma (PMID: 26599282).