• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

SKIV2L Monoclonal antibody

SKIV2L Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1A9G2

Cat no : 68547-1-Ig

Print datasheet

Synonyms

170A, DDX13, Helicase like protein, Helicase SKI2W, HLP, SKI2, SKI2W, SKIV2, SKIV2L, W

验证数据展示

  • WB analysis using 68547-1-Ig

    WB analysis using 68547-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68547-1-Ig (SKIV2L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells, Jurkat cells, MOLT-4 cells, NK-92 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68547-1-Ig targets SKIV2L in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen SKIV2L fusion protein Ag33679 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name superkiller viralicidic activity 2-like (S. cerevisiae)
Synonyms 170A, DDX13, Helicase like protein, Helicase SKI2W, HLP, SKI2, SKI2W, SKIV2, SKIV2L, W
Calculated Molecular Weight 1246 aa, 138 kDa
Observed Molecular Weight138-145 kDa
GenBank Accession NumberBC015758
Gene Symbol SKIV2L
Gene ID (NCBI) 6499
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15477
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The Ski complex is a multiprotein complex required for exosome-mediated RNA surveillance, including the regulation of normal mRNA and the decay of nonfunctional mRNA. Component of the SKI complex which is thought to be involved in exosome-mediated RNA decay and associates with transcriptionally active genes in a manner dependent on PAF1 complex (PAF1C) [PMID:22444670]. SKIV2L , one component of the Ski complex, acts as an RNA helicase with a role in exosome recruitment or activation. It is involved in the degradation of RNAs by the exosome and may have a role in autophagy [PMID:11719186].

实验方案

Product Specific Protocols
WB protocol for SKIV2L antibody 68547-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols