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验证数据展示

  • WB analysis using 68547-1-Ig

    WB analysis using 68547-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68547-1-Ig (SKIV2L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 68547-1-Ig

    IHC staining of human placenta using 68547-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68547-1-Ig (SKIV2L antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SKIV2L antibody included
  • IHC staining of human placenta using 68547-1-Ig

    IHC staining of human placenta using 68547-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68547-1-Ig (SKIV2L antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SKIV2L antibody included

SKIV2L Monoclonal antibody

SKIV2L Monoclonal Antibody for WB, IHC, ELISA
Cat No. 68547-1-Ig

产品说明书

CloneNo. 1A9G2

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, IHC, ELISA

别名

HLP, Helicase SKI2W, EC:3.6.4.13, DDX13, 1A9G2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells, Jurkat cells, MOLT-4 cells, NK-92 cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy SKIV2L Ready-To-Use IHC Kit. SKIV2L primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68547-1-Ig targets SKIV2L in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 SKIV2L fusion protein Ag33679 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 superkiller viralicidic activity 2-like (S. cerevisiae)
别名 HLP, Helicase SKI2W, EC:3.6.4.13, DDX13, 1A9G2
计算分子量 1246 aa, 138 kDa
观测分子量138-145 kDa
GenBank蛋白编号BC015758
基因名称 SKIV2L
Gene ID (NCBI) 6499
RRIDAB_3085252
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ15477
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The Ski complex is a multiprotein complex required for exosome-mediated RNA surveillance, including the regulation of normal mRNA and the decay of nonfunctional mRNA. Component of the SKI complex which is thought to be involved in exosome-mediated RNA decay and associates with transcriptionally active genes in a manner dependent on PAF1 complex (PAF1C) [PMID:22444670]. SKIV2L , one component of the Ski complex, acts as an RNA helicase with a role in exosome recruitment or activation. It is involved in the degradation of RNAs by the exosome and may have a role in autophagy [PMID:11719186].

实验方案

Product Specific Protocols
WB protocol for SKIV2L antibody 68547-1-IgDownload protocol
IHC protocol for SKIV2L antibody 68547-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols