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验证数据展示

  • WB analysis using 68547-1-Ig (same clone as 68547-1-PBS)

    WB analysis using 68547-1-Ig (same clone as 68547-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68547-1-Ig (SKIV2L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68547-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 68547-1-Ig (same clone as 68547-1-PBS)

    IHC staining of human placenta using 68547-1-Ig (same clone as 68547-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68547-1-Ig (SKIV2L antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68547-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SKIV2L antibody included
  • IHC staining of human placenta using 68547-1-Ig (same clone as 68547-1-PBS)

    IHC staining of human placenta using 68547-1-Ig (same clone as 68547-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68547-1-Ig (SKIV2L antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68547-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SKIV2L antibody included

SKIV2L Monoclonal antibody, PBS Only

SKIV2L Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 68547-1-PBS

产品说明书

CloneNo. 1A9G2

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

HLP, Helicase SKI2W, EC:3.6.4.13, DDX13, 1A9G2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy SKIV2L Ready-To-Use IHC Kit. SKIV2L primary antibody included.

产品信息

68547-1-PBS targets SKIV2L in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原 SKIV2L fusion protein Ag33679 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 superkiller viralicidic activity 2-like (S. cerevisiae)
别名 HLP, Helicase SKI2W, EC:3.6.4.13, DDX13, 1A9G2
计算分子量 1246 aa, 138 kDa
观测分子量138-145 kDa
GenBank蛋白编号BC015758
基因名称 SKIV2L
Gene ID (NCBI) 6499
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ15477
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The Ski complex is a multiprotein complex required for exosome-mediated RNA surveillance, including the regulation of normal mRNA and the decay of nonfunctional mRNA. Component of the SKI complex which is thought to be involved in exosome-mediated RNA decay and associates with transcriptionally active genes in a manner dependent on PAF1 complex (PAF1C) [PMID:22444670]. SKIV2L , one component of the Ski complex, acts as an RNA helicase with a role in exosome recruitment or activation. It is involved in the degradation of RNAs by the exosome and may have a role in autophagy [PMID:11719186].