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SUCLA2 Monoclonal antibody

SUCLA2 Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2C7E4

Cat No : 68549-1-Ig

Print datasheet

Synonyms

A BETA, SCS betaA, SUCLA2

验证数据展示

  • IF Staining of Caco-2 using 68549-1-Ig

    IF Staining of Caco-2 using 68549-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using SUCLA2 antibody (68549-1-Ig, Clone: 2C7E4 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

  • WB analysis using 68549-1-Ig

    WB analysis using 68549-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68549-1-Ig (SUCLA2 antibody) at dilution of 1:9900 incubated at room temperature for 1.5 hours.

  • WB analysis using 68549-1-Ig

    WB analysis using 68549-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68549-1-Ig (SUCLA2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHepG2 cells, LNCaP cells, HeLa cells, Jurkat cells, K-562 cells, mouse brain tissue
Positive IF detected inCaco-2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunofluorescence (IF)IF : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68549-1-Ig targets SUCLA2 in WB, IF, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, IF, ELISA Application Description
Tested Reactivity Human, mouse
Immunogen SUCLA2 fusion protein Ag17967 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name succinate-CoA ligase, ADP-forming, beta subunit
Synonyms A BETA, SCS betaA, SUCLA2
Calculated Molecular Weight 463 aa, 50 kDa
Observed Molecular Weight 48-50 kDa
GenBank Accession NumberBC027587
Gene Symbol SUCLA2
Gene ID (NCBI) 8803
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9P2R7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SUCLA2, β subunit of succinate-CoA ligase, contributes to energy production in mitochondria through the succinate-CoA ligase enzyme. Succinate-CoA ligase catalyses the reversible conversion of succinyl-CoA and ADP or GDP to succinate and ATP or GTP. SUCLA2 mutations cause global protein succinylation and mitochondrial DNA depletion (PMID: 33230181, PMID: 20301762). Mutations in SUCLA2 are also associated with SUCLA2-related mitochondrial DNA depletion syndrome, leigh syndrome, down syndrome (PMID: 23010432).

实验方案

Product Specific Protocols
WB protocol for SUCLA2 antibody 68549-1-IgDownload protocol
IF protocol for SUCLA2 antibody 68549-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols