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Species Reactivity
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验证数据展示

  • IHC staining of human colon cancer using 68549-1-Ig (same clone as 68549-1-PBS)

    IHC staining of human colon cancer using 68549-1-Ig (same clone as 68549-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68549-1-Ig (SUCLA2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68549-1-PBS in a different storage buffer formulation.

  • IF Staining of Caco-2 using 68549-1-Ig (same clone as 68549-1-PBS)

    IF Staining of Caco-2 using 68549-1-Ig (same clone as 68549-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using SUCLA2 antibody (68549-1-Ig, Clone: 2C7E4 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 68549-1-PBS in a different storage buffer formulation.

  • WB analysis using 68549-1-Ig (same clone as 68549-1-PBS)

    WB analysis using 68549-1-Ig (same clone as 68549-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68549-1-Ig (SUCLA2 antibody) at dilution of 1:9900 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68549-1-PBS in a different storage buffer formulation.

  • WB analysis using 68549-1-Ig (same clone as 68549-1-PBS)

    WB analysis using 68549-1-Ig (same clone as 68549-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68549-1-Ig (SUCLA2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68549-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 68549-1-Ig (same clone as 68549-1-PBS)

    IF Staining of HepG2 using 68549-1-Ig (same clone as 68549-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SUCLA2 antibody (68549-1-Ig, Clone: 2C7E4 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red). This data was developed using the same antibody clone with 68549-1-PBS in a different storage buffer formulation.

SUCLA2 Monoclonal antibody, PBS Only

SUCLA2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 68549-1-PBS

Print datasheet

CloneNo. 2C7E4

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

SCS betaA, EC:6.2.1.5, ATP-specific succinyl-CoA synthetase subunit beta, A-SCS, A BETA

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 
100 μg

¥5800/ 100 μg

浓度: 1mg/ml

库存：1周货期

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产品信息

68549-1-PBS targets SUCLA2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen SUCLA2 fusion protein Ag17967 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name succinate-CoA ligase, ADP-forming, beta subunit
Synonyms SCS betaA, EC:6.2.1.5, ATP-specific succinyl-CoA synthetase subunit beta, A-SCS, A BETA
Calculated Molecular Weight 463 aa, 50 kDa
Observed Molecular Weight 48-50 kDa
GenBank Accession NumberBC027587
Gene Symbol SUCLA2
Gene ID (NCBI) 8803
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9P2R7
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SUCLA2, β subunit of succinate-CoA ligase, contributes to energy production in mitochondria through the succinate-CoA ligase enzyme. Succinate-CoA ligase catalyses the reversible conversion of succinyl-CoA and ADP or GDP to succinate and ATP or GTP. SUCLA2 mutations cause global protein succinylation and mitochondrial DNA depletion (PMID: 33230181, PMID: 20301762). Mutations in SUCLA2 are also associated with SUCLA2-related mitochondrial DNA depletion syndrome, leigh syndrome, down syndrome (PMID: 23010432).